| Journal: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Database: | |
| System number: | 000537086 |
| ISSN: | 0036-4665 |
| Authors: | Leonel, João Augusto Franco1 Tannihão, Bruna1 Arantes, Julia Assis1 Vioti, Geovanna1 Benassi, Julia Cristina1 Brandi, Roberta Ariboni1 Ferreira, Helena Lage1 Keid, Lara Borges1 Soares, Rodrigo Martins2 Oliveira, Trícia Maria Ferreira de Sousa1 |
| Institutions: | 1Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Pirassununga. Brasil 2Universidade de São Paulo, Faculdade de Medicina Veterinária e Zootecnia, Pirassununga. Brasil |
| Year: | 2021 |
| Volumen: | 63 |
| Country: | Brasil |
| Language: | Inglés |
| English abstract | Visceral leishmaniasis (VL) is a neglected tropical disease caused by the Leishmania infantum parasite. The protozoan is able to infect several domestic and wild mammals. Since the first report on Leishmania spp. infection in horses in South America, leishmaniasis in equids has been highlighted in Brazil. A molecular epidemiological survey was carried out to verify the occurrence of Leishmania spp. DNA in horses and donkeys, in leishmaniases endemic areas in Sao Paulo State, Brazil. To this end, blood samples were obtained from 107 horses and 36 donkeys and subjected to DNA extraction followed by PCR targeting the ITS-1 region. Among the horses and donkeys, 1.87% (2/107) and 8.33% (3/36) were positive by PCR, respectively. The DNA sequencing of the ITS-1 amplification products confirmed L. infantum DNA in these animals. Our results suggest that horses and donkeys from non-VL and VL endemic areas of São Paulo State may be infected by the parasite. |
| Keyword: | Asinines, Equines, Equids, Leishmania infantum, ITS-1, PCR |
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