| Journal: | Revista do Instituto de Medicina Tropical de Sao Paulo |
| Database: | |
| System number: | 000537127 |
| ISSN: | 0036-4665 |
| Authors: | Valença, Ian Nunes1 Bezerra, Rafael dos Santos1 Peronni, Kamila Chagas1 Sauvage, Virginie2 Vandenbogaert, Mathias3 Caro, Valérie3 Silva Junior, Wilson Araújo da1 Covas, Dimas Tadeu1 Silva Pinto, Ana Cristina1 Laperche, Syria2 Kashima, Simone1 Slavov, Svetoslav Nanev1 |
| Institutions: | 1Universidade de São Paulo, Faculdade de Medicina de Ribeirão Preto, Ribeirão Preto. Brasil 2Institut National de la Transfusion Département d’études des Agents Transmissibles par le Sang, Centre National de Référence Risques Infectieux Transfusionnels, Paris. Francia 3Unité Environnement et Risques Infectieux, Institut Pasteur, Paris. Francia |
| Year: | 2021 |
| Volumen: | 63 |
| Country: | Brasil |
| Language: | Inglés |
| English abstract | To date, blood banks apply routine diagnosis to a specific spectrum of transfusion-transmitted viruses. Even though this measure is considered highly efficient to control their transmission, the threat imposed by emerging viruses is increasing globally, which can impact transfusion safety, especially in the light of the accelerated viral discovery by novel sequencing technologies. One of the most important groups of patients, who may indicate the presence of emerging viruses in the field of blood transfusion, is the group of individuals who receive multiple transfusions due to hereditary hemoglobinopathies. It is possible that they harbor unknown or unsuspected parenterally-transmitted viruses. In order to elucidate this, nucleic acids from 30 patients with beta-thalassemia were analyzed by Illumina next-generation sequencing and bioinformatics analysis. Three major viral families: Anelloviridae, Flaviviridae and Hepadnaviridae were identified. Among them, anelloviruses were the most representative, being detected with high number of reads in all tested samples. Human Pegivirus 1 (HPgV-1, or GBV-C), Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) were also identified. HBV and HCV detection was expected due to the high seroprevalence in patients with beta thalassemia. Our results do not confirm the presence of emerging or unsuspected viruses threatening the transfusion safety at present, but can be used to actively search for viruses that threaten blood transfusion safety. We believe that the application of viral metagenomics in multiple-transfused patients is highly useful to monitor possible viral transfusion threats and for the annotation of their virome composition. |
| Keyword: | Metagenomics, Next-generation sequencing, Beta-thalassemia, Virome, Hemotherapy, Emerging viruses |
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