| Journal: | Revista de investigación clínica |
| Database: | PERIÓDICA |
| System number: | 000452959 |
| ISSN: | 0034-8376 |
| Authors: | Akinyemi, Kabiru O1 Al Khafaji, Noor S.K2 Al Alaq, Farah T2 Fakorede, Christopher O1 Al Dahmoshi, Hussein O.M2 Iwalokun, Bamidele A3 Akpabio, Imaobong1 Alkafaas, Samar Sami4 Saki, Morteza5 |
| Institutions: | 1Lagos State University, Department of Microbiology, Ojo, Lagos. Nigeria 2University of Babylon, Department of Biology, Hillah. Irak 3Nigerian Institute of Medical Research, Department of Molecular Biology and Biotechnology, Yaba, Lagos. Nigeria 4Tanta University, Tanta, Gharbia. Egipto 5Ahvaz Jundishapur University of Medical Sciences, Department of Microbiology, Ahvaz, Khuzestan. Irán |
| Year: | 2022 |
| Season: | May-Jun |
| Volumen: | 74 |
| Number: | 3 |
| Pages: | 165-171 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | There is scarce information about the occurrence of extended-spectrum β-lactamases (ESBLs) in Salmonella enterica serovar Typhi (S. Typhi) from patients with typhoid fever. Objective: To study the antimicrobial resistance and ESBL encoding genes among S. Typhi isolates in aforesaid patients from Lagos, Nigeria. Methods: S. Typhi isolates were collected from blood samples of typhoid fever patients from 4 academic medical centers in Lagos, Nigeria. The identification of isolates and their antibiotic susceptibility testing were performed by standard bacteriological techniques and disc diffusion method, respectively. The production of ESBLs was investigated using combination disk test (CDT) and polymerase chain reaction (PCR). Results: A total of 27 S. Typhi isolates was collected. All isolates were susceptible to imipenem and nitrofurantoin. Fifteen (55.6%) isolates were multidrug-resistant (MDR). The CDT test showed 11 (40.7%) ESBL producer isolates. However, the PCR revealed a higher occurrence rate for ESBL producers (66.7%, n = 18/27). The ESBL genes were as follows: blaCTX-M (37.0%, n = 10/27), blaSHV (18.5%, n = 5/27), and blaTEM (44.4%, n = 12/27). All ESBL positive S. Typhi isolates were MDR. Conclusions: This study showed the emergence of ESBL-harboring S. Typhi in patients with typhoid fever from Nigeria |
| Disciplines: | Medicina |
| Keyword: | Farmacología, Microbiología, Resistencia a fármacos, Beta-lactamasa, Salmonella enterica |
| Keyword: | Pharmacology, Microbiology, Drug resistance, Beta-lactamase, Salmonella enterica |
| Full text: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
