Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Document title: Viability and molecular authentication of Coccidioides immitis strains from culture collection of the Instituto Oswaldo Cruz, Rio de Janeiro, Brazil
Journal: Revista da Sociedade Brasileira de Medicina Tropical
Database: PERIÓDICA
System number: 000282830
ISSN: 0037-8682
Authors: 1
2
Institutions: 1Fundacao Oswaldo Cruz, Instituto de Pesquisa Clinica Evandro Chagas, Rio de Janeiro. Brasil
2Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil
Year:
Season: May-Jun
Volumen: 39
Number: 3
Pages: 241-244
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract Twenty Coccidioides immitis strains were evaluated. Only 5 of the 20 strains kept under mineral oil maintained their viability while all 5 subcultures preserved in water remained viable and none of the 13 subcultures kept in soil were viable. A 519 bp PCR product from the csa gene confirmed the identity of the strains
Portuguese abstract Vinte cepas de Coccidioides immitis foram avaliadas. Cinco das 20 cepas preservadas sob óleo mineral mantiveram-se viáveis, todas as 5 subculturas preservadas em água permaneceram viáveis e nenhuma das 13 subculturas mantidas em solo foi viável. Um produto de PCR de 519 pb do gene csa confirmou a identidade das cepas
Disciplines: Biología,
Medicina
Keyword: Hongos,
Parasitología,
Microbiología,
Coccidioides immitis,
Cepas,
Preservación,
PCR
Keyword: Biology,
Medicine,
Fungi,
Parasitology,
Microbiology,
Preservation,
PCR,
Coccidioides immitis,
Strains
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