| Revista: | Revista brasileira de ciencia avicola |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000279319 |
| ISSN: | 1516-635X |
| Autores: | Oliveira, Glaucia Helaine de1 Berchieri-Junior, Angelo Montassier, Helio Jose |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil |
| Año: | 2006 |
| Periodo: | Ene-Mar |
| Volumen: | 8 |
| Número: | 1 |
| Paginación: | 51-54 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Nota breve o noticia |
| Enfoque: | Experimental |
| Resumen en inglés | This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis |
| Disciplinas: | Medicina veterinaria y zootecnia |
| Palabras clave: | Aves de corral, Medicina veterinaria, Pollos, Detección, Salmonella, ELISA, Enzimas, Serología |
| Keyword: | Veterinary medicine and animal husbandry, Poultry, Veterinary medicine, Chicken, Detection, Salmonella, ELISA, Enzymes, Serology |
| Texto completo: | Texto completo (Ver HTML) |
