| Journal: | Revista brasileira de ciencia avicola |
| Database: | PERIÓDICA |
| System number: | 000279319 |
| ISSN: | 1516-635X |
| Authors: | Oliveira, Glaucia Helaine de1 Berchieri-Junior, Angelo Montassier, Helio Jose |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil |
| Year: | 2006 |
| Season: | Ene-Mar |
| Volumen: | 8 |
| Number: | 1 |
| Pages: | 51-54 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Experimental |
| English abstract | This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Aves de corral, Medicina veterinaria, Pollos, Detección, Salmonella, ELISA, Enzimas, Serología |
| Keyword: | Veterinary medicine and animal husbandry, Poultry, Veterinary medicine, Chicken, Detection, Salmonella, ELISA, Enzymes, Serology |
| Full text: | Texto completo (Ver HTML) |
