| Journal: | Quimica nova |
| Database: | PERIÓDICA |
| System number: | 000196510 |
| ISSN: | 0100-4042 |
| Authors: | Luca, Gilmara C1 Reis, Boaventura F2 |
| Institutions: | 1Universidade de Sao Paulo, Instituto de Quimica de Sao Carlos, Sao Carlos, Sao Paulo. Brasil 2Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, Sao Paulo. Brasil |
| Year: | 2001 |
| Season: | Mar-Abr |
| Volumen: | 24 |
| Number: | 2 |
| Pages: | 191-194 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | A spectrophotometric flow injection analysis (FIA) procedure employing natural urease enzyme source for the determination of urea in animal blood plasma was developed. Among leguminous plants used in the Brazilian agriculture, the Cajanus cajan specie was selected as urease source considering its efficiency and availability. A minicolumn was filled with leguminous fragments and coupled to the FIA manifold, where urea was on-line converted to ammonium ions and subsequently it was quantified by spectrophotometry. The system was employed to determine urea in animal plasma samples without any prior treatment. Accuracy was assessed by comparison results with those obtained employing the official procedure and no significant difference at 90 % confidence level was observed. Other profitable features such as an analytical throughput of 30 determinations per hour, a reagent consumption of 19.2 mg sodium salicylate, 0.5 mg sodium hipochloride and a relative standard deviation of 1.4 % (n= 12) were also obtained |
| Disciplines: | Medicina, Química |
| Keyword: | Diagnóstico, Química analítica, Ureasa, Enzimas, Urea, Plasma sanguíneo, Cajanus cajan, Análisis de flujo, Espectrofotometría |
| Keyword: | Medicine, Chemistry, Diagnosis, Analytical chemistry, Urease, Enzymes, Urea, Blood plasma, Cajanus cajan, Flow analysis, Spectrophotometry |
| Full text: | Texto completo (Ver HTML) |
