| Journal: | Quimica nova |
| Database: | PERIÓDICA |
| System number: | 000313255 |
| ISSN: | 0100-4042 |
| Authors: | Alves, Sandriel Trindade1 Dias, Rafael Carlos Eloy Benassi, Marta de Toledo Scholz, Maria Brigida dos Santos2 |
| Institutions: | 1Universidade Estadual de Londrina, Departamento de Tecnologia de Alimentos e Medicamentos, Londrina, Parana. Brasil 2Instituto Agronomico do Parana, Londrina, Parana. Brasil |
| Year: | 2006 |
| Season: | Nov |
| Volumen: | 29 |
| Number: | 6 |
| Pages: | 1164-1168 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A reverse phase liquid chromatography method was developed for simultaneous determination of trigonelline, caffeine, nicotinic and chlorogenic (5-CQA) acids in roasted coffee. A gradient of acetic acid/acetonitrile was used as mobile phase and detection was carried out in the UV. The samples were extracted with acetonitrile/water (5:95 v/v) at 80 ºC/10 min. Good recovery (89 to 104%), repeatability and linearity were obtained. Detection limits of 0.01, 0.15, 0.04 and 0.04 mg mL-1 were observed for nicotinic acid, trigonelline, 5-CQA and caffeine. The method, applied to arabica and robusta coffees with different degrees of roasting, was efficient and fast (~35 min) and also allowed identification of cinnamic acids |
| Disciplines: | Química |
| Keyword: | Química de alimentos, Café tostado, Trigonelina, Cafeína, Acido nicotínico, Acido clorogénico, Cromatografía líquida de alta resolución (HPLC), Coffea canephora, Coffea arabica |
| Keyword: | Chemistry, Food chemistry, Roasted coffee, Trigonelline, Caffeine, Nicotinic acid, Chlorogenic acid, High performance liquid chromatography (HPLC), Coffea arabica, Coffea canephora |
| Full text: | Texto completo (Ver HTML) |
