| Journal: | Quimica nova |
| Database: | PERIÓDICA |
| System number: | 000313206 |
| ISSN: | 0100-4042 |
| Authors: | Trevisan, Henrique Celso1 Medeiros, Joao Batista de Lisboa, Helen Cristina Favero |
| Institutions: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Quimica, Araraquara, Sao Paulo. Brasil |
| Year: | 2006 |
| Season: | Jul |
| Volumen: | 29 |
| Number: | 4 |
| Pages: | 865-867 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A simple, fast and low-cost methodology was optimized, seeking preparation of a crude pig liver esterase (PLE) concentrate. Basically, the method consisted of the following steps: liver homogenization, acetone washing, enzyme extraction and purification/concentration. Starting from 1 kg of fresh liver more than 200 kU of PLE suspension were obtained after 8 hours, at an estimated cost of US |
| Disciplines: | Química |
| Keyword: | Bioquímica, Esterasas, Hígado, Cerdos, Extracción química, Biocatálisis |
| Keyword: | Chemistry, Biochemistry, Esterases, Liver, Pigs, Chemical extraction, Biocatalysis |
| Full text: | Texto completo (Ver HTML) |
