Evaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation



Document title: Evaluation of peanut genotypes for resistance to Tomato spotted wilt virus by mechanical and thrips inoculation
Journal: Pesquisa agropecuaria brasileira = Brazilian journal of agricultural research
Database: PERIÓDICA
System number: 000301068
ISSN: 0100-204X
Authors: 1

2
3
4
5
Institutions: 1Universidade Federal da Paraiba, Departamento de Fitotecnia, Areia, Paraiba. Brasil
2Udon Thani Rajabhat University, Faculty of Technology, Udon Thani. Tailandia
3Agdia Incorporated, Elkhart, Indiana. Estados Unidos de América
4Universidade Federal de Pernambuco, Departamento de Agronomia, Recife, Pernambuco. Brasil
5University of Georgia, Department of Plant Pathology, Athens, Georgia. Estados Unidos de América
Year:
Season: Jun
Volumen: 41
Number: 6
Pages: 937-942
Country: Brasil
Language: Inglés
Document type: Nota breve o noticia
Approach: Experimental
English abstract The objective of this work was to evaluate the reactions of three peanut breeding lines (IC-10, IC-34, and ICGV 86388) to Tomato spotted wilt virus (TSWV) by mechanical and thrips inoculation, under greenhouse conditions, and compare them to the reactions of cultivars SunOleic, Georgia Green, and the breeding line C11-2-39. TSWV infection by mechanical inoculation was visually assessed using an index ranging from 0 (no symptoms) to 4 (apical death). Enzyme-linked immunosorbent assay was used to confirm TSWV infection from both mechanical and thrips inoculations. IC-10, IC-34, ICGV 86388, and C11-2-39 were more resistant than the cultivars SunOleic and Georgia Green based on mechanical inoculation. Upon thrips inoculation only IC-34 and ICGV-86388 were infected by TSWV, as demonstrated by reverse transcription polymerase chain reaction (RT-PCR), although no symptoms of infection were observed. The peanut breeding lines IC-10, IC-34, and ICGV 86388 show higher level of resistance to TSWV than cultivar Georgia Green considered a standard for TSWV resistance
Portuguese abstract O objetivo deste trabalho foi avaliar as reações das linhagens de amendoim IC-10, IC-34 e ICGV 86388 quanto à resistência ao Tomato spotted wilt virus (TSWV), por meio de inoculação mecânica e por tripes, em casa de vegetação, e compará-las às reações das cultivares SunOleic, Georgia Green e da linhagem C11-2-39. A infecção de TSWV após inoculação mecânica foi visualmente avaliada, utilizando-se escala de notas que variam de zero (sem sintomas) a quatro (morte apical). A análise pelo teste ELISA confirmou a infecção de TSWV em ambos os tipos de inoculação. As linhagens IC-10, IC-34, ICGV 86388 e C11-2-39 foram mais resistentes do que as cultivares SunOleic e Georgia Green, com base nos resultados da inoculação mecânica. Em relação à inoculação por tripes, apenas IC-34 e ICGV 86388 foram infectadas por TSWV, conforme demonstrado pela reação em cadeia da polimerase com transcrição reversa (RT-PCR), embora nenhum sintoma de infecção tenha sido observado. As linhagens IC-10, IC-34 e ICGV 86388 mostram maior resistência ao vírus, quando comparadas à Georgia Green, considerada padrão de resistência ao TSWV
Disciplines: Agrociencias
Keyword: Fitopatología,
Cacahuate,
Arachis hypogaea,
Genotipos,
Resistencia,
Virus,
Inoculación
Keyword: Agricultural sciences,
Phytopathology,
Peanut,
Arachis hypogaea,
Genotypes,
Resistance,
Virus,
Inoculation
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