| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000420025 |
| ISSN: | 0074-0276 |
| Authors: | Batista, Cassiano Martin1 Saad, Felipe1 Ceccoti, Stephane Pini Costa1 Eger, Iriane2 Soares, Maurilio José1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Carlos Chagas, Curitiba, Parana. Brasil 2Universidade Estadual de Ponta Grossa, Departamento de Biologia Geral, Ponta Grossa, Parana. Brasil |
| Year: | 2018 |
| Volumen: | 113 |
| Number: | 8 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Dynamic S-palmitoylation of proteins is the addition of palmitic acid by zDHHC palmitoyl transferases (PATs) and depalmitoylation by palmitoyl protein thioesterases (PPTs). A putative PAT (TcPAT1) has been previously identified in Trypanosoma cruzi, the etiological agent of Chagas disease. Here we analyse other 14 putative TcPATs and 2 PPTs in the parasite genome. T. cruzi cell lines expressing TcPATs and TcPPTs plus a FLAG tag at the C terminus were produced for most enzymes, with positive detection by indirect immunofluorescence. Overexpressed TcPATs were mostly found as single spots at the parasite anterior end, while the TcPPTs were dispersed throughout the parasite body |
| Disciplines: | Biología, Química |
| Keyword: | Protozoarios, Bioquímica, Proteínas, Acido palmítico, Palmitoilación dinámica, Trypanosoma cruzi |
| Keyword: | Protozoa, Biochemistry, Proteins, Palmitic acid, Dynamic palmitoylation, Trypanosoma cruzi |
| Full text: | Texto completo (Ver HTML) |
