| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000413840 |
| ISSN: | 0074-0276 |
| Authors: | Figueiredo, Webertty Mayk Eufrasio1 Viana, Sayonara de Melo1 Alves, Dorotheia Teixeira1 Guerra, Priscila Valera1 Coelho, Zirlane Castelo Branco2 Barbosa, Helene Santos3 Teixeira, Maria Jania1 |
| Institutions: | 1Universidade Federal do Ceara, Faculdade de Medicina, Fortaleza, Ceara. Brasil 2Universidade Federal do Ceara, Faculdade de Farmacia, Fortaleza, Ceara. Brasil 3Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil |
| Year: | 2017 |
| Season: | Ago |
| Volumen: | 112 |
| Number: | 8 |
| Pages: | 561-568 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Visceral leishmaniasis (VL) caused by Leishmania infantum is characterised by the loss of the ability of the host to generate an effective immune response. Chemokines have a direct involvement in the pathogenesis of leishmaniasis, causing a rapid change in the expression of these molecules during infection by Leishmania. OBJECTIVES Herein, it was investigated the role of CXCL10 in controlling infection by L. infantum. METHODS RAW 264.7 macrophages were infected with L. infantum in vitro and treated or not with CXCL10 (25, 50 and 100 ng/mL). Parasite load, as well as nitric oxide (NO), IL-4 and IL-10 production were assessed at 24 and 48 h after infection. In vivo, BALB/c mice were infected and treated or not with CXCL10 (5 μg/kg) at one, three and seven days of infection. Parasite load, IFN-g, IL-4, TGF-β and IL-10 were evaluated one, seven and 23 days post treatment. FINDINGS In vitro, CXCL10 reduced parasitic load, not dependent on NO, and inhibited IL-10 and IL-4 secretion. In vivo, CXCL10 was able to reduce the parasite load in both liver and spleen, four weeks after infection, representing a higher decrease in the number of parasites in these organs, also induced IFN-γ at day 23 after treatment, correlating with the decrease in parasite load, and reduced IL-10 and TGF-β. MAIN CONCLUSIONS This study suggests a partial protective role of CXCL10 against L. infantum, mediated by IFN-g, not dependent on NO, and with suppression of IL-10 and TGF-β. These data may provide information for the development of new approaches for future therapeutic interventions for VL |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Inmunología, Leishmaniasis visceral, Respuesta inmune, Quimocinas, Oxido nítrico, Macrófagos |
| Keyword: | Medicine, Parasitology, Immunology, Visceral leishmaniasis, Immune response, Chemokines, Nitric oxide, Macrophages |
| Full text: | Texto completo (Ver HTML) |
