| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000420023 |
| ISSN: | 0074-0276 |
| Authors: | Pinto, Lysianne1 Granja, Luiz Fernando Zmetek1 Almeida, Mariana Amorim de2 Alviano, Daniela Sales1 Silva, Maria Helena da1 Ejzemberg, Regina1 Rozental, Sonia3 Alviano, Celuta Sales1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Instituto de Microbiologia "Paulo de Goes", Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Instituto de Macromoleculas Professora Eloisa Mano, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil |
| Year: | 2018 |
| Volumen: | 113 |
| Number: | 8 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Melanin production has been associated with virulence in various pathogenic fungi, including Fonsecaea pedrosoi, the major etiological agent for chromoblastomycosis, a subcutaneous fungal disease that occurs in South America. OBJECTIVE The aim of this study was to evaluate the effects of acid-basic extracted F. pedrosoi melanin particles and fungal cell ghosts obtained by Novozym 234 treatment on their ability to activate the human complement system. METHODS The ability of melanin particles and fungal cell ghosts to activate the human complement system was evaluated by complement consumption, immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Unsensitised melanin particles and melanin ghosts presented complement consumption of 82.67 ± 2.08% and 96.04 ± 1.13%, respectively. Immunofluorescence assays revealed intense deposition of the C3 and C4 fragments on the surface of melanin particles and ghosts extracted from F. pedrosoi. Deposition of the C3, C4, and C5 fragments onto melanin samples and zymosan was confirmed by ELISA. Deposition of small amounts of C1q and C9 onto melanin samples and zymosan was detected by ELISA. CONCLUSION Fonsecaea pedrosoi melanin particles and fungal cell ghosts activated the complement system mainly through an alternative pathway |
| Disciplines: | Medicina |
| Keyword: | Hongos, Dermatología, Inmunología, Cromoblastomicosis, Melanina, Respuesta inmune, Complemento, Fonsecaea pedrosoi |
| Keyword: | Fungi, Dermatology, Immunology, Chromoblastomycosis, Melanin, Immune response, Complement, Fonsecaea pedrosoi |
| Full text: | Texto completo (Ver HTML) |
