Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil



Document title: Lack of serological and molecular evidences of Zika virus circulation in non-human primates in three states from Brazil
Journal: Memorias do Instituto Oswaldo Cruz
Database: PERIÓDICA
System number: 000452391
ISSN: 0074-0276
Authors: 1
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Institutions: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biotecnologia, Botucatu, Sao Paulo. Brasil
2Universidade Federal do Parana, Departamento de Ciencias Veterinarias, Palotina, Parana. Brasil
3Faculdade de Medicina de Sao Jose do Rio Preto, Laboratorio de Pesquisas em Virologia, Sao Jose do Rio Preto, Sao Paulo. Brasil
4Universidade Federal de Campina Grande, Patos, Paraíba. Brasil
5Refugio Biologico Bela Vista, Itaipu Binacional, Foz do Iguacu, Parana. Brasil
6Zoologico Bosque do Guarani, Foz do Iguacu, Parana. Brasil
7Zoologico Municipal Quinzinho de Barros, Sorocaba, Sao Paulo. Brasil
8Universidade Federal da Paraiba, Departamento de Ciencias Veterinarias, Areia, Paraiba. Brasil
9Universidade Federal da Integracao Latino-Americana, Foz do Iguacu, Parana. Brasil
Year:
Volumen: 117
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Zika virus (ZIKV) was discovered in 1947 with the virus isolation from Rhesus monkey (Macaca mulatta) in Uganda forest, Africa. Old World Primates are involved in a sylvatic cycle of maintenance of this arbovirus, however a limited knowledge about the role of New World primates in ZIKV transmission cycles has been established. OBJECTIVE This work aimed to investigate the presence of enzootic circulation of ZIKV in New World Primates from three Brazilian states: São Paulo, Paraíba, and Paraná. METHODS We analyzed 100 non-human primate samples collected in 2018 and 2020 from free-ranging and captive environments from São Paulo (six municipalities belonging to Sorocaba region), Paraíba (João Pessoa municipality), and Paraná (Foz do Iguaçu municipality) using reverse transcriptase quantitative polymerase reaction (RT-qPCR) assays, indirect enzyme-linked immunosorbent assay (ELISA), and plaque reduction neutralization test (PRNT). FINDINGS All samples (n = 141) tested negative for the presence of ZIKV genome from tissue and blood samples. In addition, all sera (n = 58) from Foz do Iguaçu’ non-human primates (NHPs) were negative in serological assays. MAIN CONCLUSION No evidence of ZIKV circulation (molecular and serological) was found in neotropical primates. In addition, the absence of antibodies against ZIKV suggests the absence of previous viral exposure of NHPs from Foz do Iguaçu-PR
Disciplines: Medicina
Keyword: Salud pública,
Virus,
Epidemiología,
Virus del Zika,
Ciclo de transmisión
Keyword: Public health,
Virus,
Epidemiology,
Zika virus,
Transmission cycle
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