| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452378 |
| ISSN: | 0074-0276 |
| Authors: | Nisimura, Lindice Mitie1 Ferreira, Roberto Rodrigues1 Coelho, Laura Lacerda1 Souza, Elen Mello de1 Gonzaga, Beatriz Matheus1 Ferrão, Patrícia Mello1 Waghabi, Mariana Caldas1 Mesquita, Liliane Batista de1 Pereira, Mirian Claudia de Souza1 Moreira, Otacilio da Cruz1 Lannes Vieira, Joseli1 Garzoni, Luciana Ribeiro1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Angiogenesis has been implicated in tissue injury in several noninfectious diseases, but its role in Chagas disease (CD) physiopathology is unclear. OBJECTIVES The present study aimed to investigate the effect of Trypanosoma cruzi infection on cardiac angiogenesis during the acute phase of experimental CD. METHODS The signalling pathway involved in blood vessel formation and cardiac remodelling was evaluated in Swiss Webster mice infected with the Y strain of T. cruzi. The levels of molecules involved in the regulation of angiogenesis, such as vascular endothelial growth factor-A (VEGF-A), Flk-1, phosphorylated extracellular-signal-regulated protein kinase (pERK), hypoxia-inducible factor-1α (HIF-1α), CD31, α-smooth muscle actin (α-SMA) and also the blood vessel growth were analysed during T. cruzi infection. Hearts were analysed using conventional histopathology, immunohistochemistry and western blotting. FINDINGS In this study, our data demonstrate that T. cruzi acute infection in mice induces exacerbated angiogenesis in the heart and parallels cardiac remodelling. In comparison with noninfected controls, the cardiac tissue of T. cruzi-infected mice presented higher levels of (i) HIF-1α, VEGF-A, Flk-1 and pERK; (ii) angiogenesis; (iii) α-SMA+ cells in the tissue; and (iv) collagen -1 deposition around blood vessels and infiltrating throughout the myocardium. MAIN CONCLUSIONS We observed cardiac angiogenesis during acute experimental T. cruzi infection parallels cardiac inflammation and remodelling |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Infección experimental, Enfermedad de Chagas, Angiogénesis, Remodelación cardiaca |
| Keyword: | Parasitology, Experimental infection, Chagas disease, Angiogenesis, Cardiac remodelling |
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