| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452388 |
| ISSN: | 0074-0276 |
| Authors: | Almeida Silva, Fernando1 Bernardes Engemann, Andrea Reis1 Bérenger, Ana Luiza Rangel2 da Silva, Vagner Pereira2 Figueiredo, Maria Raquel2 Freitas, Dayvison Francis Saraiva1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro. Brasil 2Fundacao Oswaldo Cruz, Instituto de Tecnologia em Farmacos, Rio de Janeiro. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Sporothrix brasiliensis is the causative agent of zoonotic cases of sporotrichosis in Brazil and is associated with atypical and severe presentations in cats, dogs, and humans. Sporotrichosis treatment is usually time- and cost-consuming, sometimes with poor response and host toxicity. Schinus terebinthifolius has proven efficacy against bacteria and fungi of clinical interest. OBJECTIVE To determine the in vitro activity of S. terebinthifolius against S. brasiliensis. METHODS Five S. brasiliensis isolates and three reference strains were subjected to a hydroethanol extract derived from the leaves of S. terebinthifolius and its fractions. The minimal inhibitory concentration (MIC) was determined using the broth microdilution method according to the M38-A2 CLSI guidelines. Also, the fungicidal/fungistatic activity of the extract and fractions was studied. FINDINGS The crude extract of S. terebinthifolius inhibited the growth of S. brasiliensis (MIC: 0.5-1.0 µg/mL), while the partitioned extracts dichloromethane, ethyl acetate, and butanol demonstrated growth inhibition at 8 µg/mL due to a fungistatic activity. MAIN CONCLUSIONS Due to its in vitro efficacy against S. brasiliensis and its known pharmacological safety, S. terebinthifolius is a candidate to be tested using in vivo models of sporotrichosis |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Farmacología, Plantas medicinales, Antifúngicos, Bioensayos, Schinus terebinthifolius, Anacardiaceae, Esporotricosis, Sporothrix brasiliensis |
| Keyword: | Microbiology, Pharmacology, Medicinal plants, Sporothrix brasiliensis, Sporotrichosis, Antifungal agents, Bioassays, Schinus terebinthifolius, Anacardiaceae |
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