| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452387 |
| ISSN: | 0074-0276 |
| Authors: | Melo, Leyva Cecília Vieira de1 Souza, Felipe Corrêa Rezende de1 Baccin, Amanda de Oliveira1 Mota, Dan Jessé Gonçalves da3 Pereira Chioccola, Vera Lucia2 Pinto, Pedro Luiz Silva1 |
| Institutions: | 1Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Sao Paulo. Brasil 2Secretaria de Saude do Estado de Sao Paulo, Coordenadoria do Controle de Doencas, Sao Paulo. Brasil 3Unidade de Vigilancia em Saude, Sao Paulo. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Angiostrongylus cantonensis is the etiological agent of neuroangiostrongyliasis in humans, which is developed in gastropods and vertebrate species, mainly rodents. Human transmission occurs through consumption of molluscs and paratenic hosts infected with L3, and the migration of larvae to the central nervous system causes eosinophilic meningitis. Laboratory diagnosis is based on molecular and immunological tests, using young or adult females as a source of antigens. However, these tests give positive results only after several weeks of symptoms onset and also cross-reactions with others parasite infections may occur. OBJECTIVES The purpose of this work was to study different antigenic preparations of distinct evolutionary phases of A. cantonensis, in order to improve serological techniques for disease immunodiagnosis. METHODS For this purpose, antigenic fractions of different evolutionary forms were evaluated by Dot-enzyme-linked immunosorbent assay (Dot-ELISA) and Western blot using serum samples. FINDINGS All analysed fractions showed reactivity with serum samples from patients with neuroangiostrongyliasis, especially female membrane alkaline (FAM) and female soluble alkaline (FAS) fractions together with female soluble saline (FSS), improving the technique specificity. MAIN CONCLUSIONS The results point to the possibility of use of raw female antigens in association with alkaline membrane antigens extracted from adult worms to aid in diagnosis and helps initiate neuroangiostrongyliasis surveillance and control actions |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Inmunología, Diagnóstico, Meningitis, Inmunodiagnóstico, Eosinofilia, Angiostrongylus cantonensis |
| Keyword: | Parasitology, Immunology, Diagnosis, Meningitis, Immunodiagnosis, Eosinophilia, Angiostrongylus cantonensis |
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