| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452382 |
| ISSN: | 0074-0276 |
| Authors: | Souza, Thiago Kury Moreno de1 Westphalen, Elizabeth Visone Nunes1 Westphalen, Sansão da Rocha1 Taniguchi, Helena Hilomi1 Elias, Carlos Roberto1 Motoie, Gabriela1 Gava, Ricardo1 Pereira Chioccola, Vera Lucia1 Novaes, Christina Terra Gallafrio2 Carvalho, Noêmia Barbosa2 Bocchi, Edimar Alcides2 Cruz, Fátima das Dores da2 Rocha, Mussya Cisotto2 Shinjo, Samuel Katsuyuki2 Shikanai Yasuda, Maria Aparecida2 Ortiz, Paola Andrea3 Teixeira, Marta Maria Geraldes3 Tolezano, José Eduardo1 |
| Institutions: | 1Instituto Adolfo Lutz, Centro de Parasitologia e Micologia, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina, Sao Paulo. Brasil 3Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Sao Paulo. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Trypanosoma cruzi shows an exuberant genetic diversity. Currently, seven phylogenetic lineages, called discrete typing units (DTUs), are recognised: TcI-TcVI and Tcbat. Despite advances in studies on T. cruzi and its populations, there is no consensus regarding its heterogeneity. OBJECTIVES This study aimed to perform molecular characterisation of T. cruzi strains, isolated in the state of São Paulo, to identify the DTUs involved and evaluate their genetic diversity. METHODS T. cruzi strains were isolated from biological samples of chronic chagasic patients, marsupials and triatomines through culture techniques and subjected to molecular characterisation using the fluorescent fragment length barcoding (FFLB) technique. Subsequently, the results were correlated with complementary information to enable better discrimination between the identified DTUs. FINDINGS It was possible to identify TcI in two humans and two triatomines; TcII/VI in 19 humans, two marsupials and one triatomine; and TcIII in one human host, an individual that also presented a result for TcI, which indicated the possibility of a mixed infection. Regarding the strains characterised by the TcII/VI profile, the correlation with complementary information allowed to suggest that, in general, these parasite populations indeed correspond to the TcII genotype. MAIN CONCLUSIONS The TcII/VI profile, associated with domestic cycles and patients with chronic Chagas disease, was the most prevalent among the identified DTUs. Furthermore, the correlation of the study results with complementary information made it possible to suggest that TcII is the predominant lineage of this work |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Genética, Enfermedad de Chagas, Huésped-parásito, Trypanosoma cruzi, Diversidad genética, Biología molecular |
| Keyword: | Parasitology, Genetics, Chagas disease, Host-parasite, Trypanosoma cruzi, Genetic diversity, Molecular biology |
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