Journal: | Memorias do Instituto Oswaldo Cruz |
Database: | PERIÓDICA |
System number: | 000392265 |
ISSN: | 0074-0276 |
Authors: | Peralta, Regina Helena Saramago1 Velásquez, Jorge Néstor2 Cunha, Flavia de Souza1 Pantano, María Laura3 Sodre, Fernando Campos1 Silva, Sidnei da4 Astudillo, Osvaldo Germán3 Peralta, Jose Mauro5 Carnevale, Silvana3 |
Institutions: | 1Universidade Federal Fluminense, Faculdade de Medicina, Niteroi, Rio de Janeiro. Brasil 2Hospital Municipal de Enfermedades Infecciosas "Dr. Francisco J. Muñiz", Buenos Aires. Argentina 3Instituto Nacional de Enfermedades Infecciosas, Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán", Buenos Aires. Argentina 4Fundacao Oswaldo Cruz, Instituto Nacional de Infectologia Evandro Chagas, Rio de Janeiro. Brasil 5Universidade Federal do Rio de Janeiro, Instituto de Microbiologia "Paulo de Goes", Rio de Janeiro. Brasil |
Year: | 2016 |
Season: | Ene |
Volumen: | 111 |
Number: | 1 |
Pages: | 30-36 |
Country: | Brasil |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | The identification and characterisation of Cryptosporidium genotypes and subtypes are fundamental to the study of cryptosporidiosis epidemiology, aiding in prevention and control strategies. The objective was to determine the genetic diversity of Cryptosporidium in samples obtained from hospitals of Rio de Janeiro, Brazil, and Buenos Aires, Argentina. Samples were analysed by microscopy and TaqMan polymerase chain reaction (PCR) assays for Cryptosporidium detection, genotyped by nested-PCR-restriction fragment length polymorphism (RFLP) analysis of the 18S rRNA gene and subtyped by DNA sequencing of the gp60 gene. Among the 89 samples from Rio de Janeiro, Cryptosporidium spp were detected in 26 by microscopy/TaqMan PCR. In samples from Buenos Aires,Cryptosporidium was diagnosed in 15 patients of the 132 studied. The TaqMan PCR and the nested-PCR-RFLP detected Cryptosporidium parvum, Cryptosporidium hominis, and co-infections of both species. In Brazilian samples, the subtypes IbA10G2 and IIcA5G3 were observed. The subtypes found in Argentinean samples were IbA10G2, IaA10G1R4, IaA11G1R4, and IeA11G3T3, and mixed subtypes of Ia and IIa families were detected in the co-infections. C. hominis was the species more frequently detected, and subtype family Ib was reported in both countries. Subtype diversity was higher in Buenos Aires than in Rio de Janeiro and two new subtypes were described for the first time |
Disciplines: | Medicina |
Keyword: | Diagnóstico, Microbiología, Protozoarios, Genotipificación, Diversidad genética, Cryptosporidium hominis, Cryptosporidium parvum |
Keyword: | Medicine, Diagnosis, Microbiology, Protozoa, Genotyping, Genetic diversity, Cryptosporidium hominis, Cryptosporidium parvum |
Full text: | Texto completo (Ver HTML) |