| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000446491 |
| ISSN: | 0074-0276 |
| Authors: | Abreu, Fabiano C.P1 Mota, Ester Alves1 Pereira, Roberta V1 Oliveira, Victor F1 Costa, Marcela P1 Gomes, Matheus de S2 Jannotti Passos, Liana K3 Borges, William C1 Guerra Sá, Renata1 |
| Institutions: | 1Universidade Federal de Ouro Preto, Nucleo de Pesquisas em Ciencias Biologicas, Ouro Preto, Minas Gerais. Brasil 2Universidade Federal de Uberlandia, Instituto de Genetica e Bioquimica, Patos de Minas, Minas Gerais. Brasil 3Fundacao Oswaldo Cruz, Centro de Pesquisas Rene Rachou, Belo Horizonte, Minas Gerais. Brasil |
| Year: | 2021 |
| Volumen: | 116 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | BACKGROUND Schistosomiasis is a disease caused by Schistosoma. Due to its complex life cycle, evolutionary position and sexual dimorphism, schistosomes have several mechanisms of gene regulation. MicroRNAs (miRNAs) are short endogenous RNAs that regulate gene expression at the post-transcriptional level by targeting mRNA transcripts. OBJECTIVES Here, we tested 12 miRNAs and identified their putative targets using a computational approach. METHODS We performed the expression profiles of a set of miRNAs and their putative targets during the parasite’s life cycle by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). FINDINGS Our results showed differential expression patterns of the mature miRNAs sma-miR-250; sma-miR-92a; sma-miR-new_4-3p; sma-miR-new_4-5p; sma-miR-new_5-5p; sma-miR-new_12-5p; sma-miR-new_13-3p and sma-miR-new_13-5p. Interestingly, many of the putative target genes are linked to oxidative phosphorylation and are up-regulated in adult-worms, which led us to suggest that miRNAs might play important roles in the post-transcriptional regulation of genes related to energetic metabolism inversion during parasite development. It is noteworthy that the expression of sma-miR-new_13-3p exhibited a negative correlation on SmNADH:ubiquinone oxidoreductase complex I. MAIN CONCLUSIONS Our analysis revealed putative miRNA genes related to important biological processes, such as transforming growth factor beta (TGF-β) signaling, proteasome regulation, glucose and lipid metabolism, immune system evasion and transcriptional regulation |
| Disciplines: | Biología |
| Keyword: | Protozoarios, Genética, ARN mitocondrial, Expresión diferencial, Genes candidatos, Schistosoma mansoni |
| Keyword: | Protozoa, Genetics, Mitochondrial RNA, Differential expression, Schistosoma mansoni, Target genes |
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