| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452397 |
| ISSN: | 0074-0276 |
| Authors: | Morais, Franciele Costa Leite1 Bello, Graziele Lima2 Costi, Cíntia3 Schmid, Karen Barros3 Soares, Tainá dos Santos1 Barcellos, Regina Bones3 Unis, Gisela4 Dias, Claudia Fontoura4 da Silva, Pedro Eduardo Almeida5 Rossetti, Maria Lucia1 |
| Institutions: | 1Universidade Luterana do Brasil, Programa de Pos-Graduacao em Biologia Celular e Molecular Aplicada a Saude, Canoas, Rio Grande do Sul. Brasil 2Instituto Nacional de Ciencia e Tecnologia em Tuberculose, Programa Institutos Nacionais de Ciencia e Tecnologia, Porto Alegre, Rio Grande do Sul. Brasil 3Secretaria da Saude do Rio Grande do Sul, Centro de Desenvolvimento Cientifico e Tecnologico, Porto Alegre, Rio Grande do Sul. Brasil 4Secretaria Estadual da Saude do Rio Grande do Sul, Hospital Sanatorio Partenon, Porto Alegre, Rio Grande do Sul. Brasil 5Universidade Federal do Rio Grande, Faculdade de Medicina, Rio Grande, Rio Grande do Sul. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | BACKGROUND Non-tuberculous mycobacteria (NTMs) cause diseases known as mycobacteriosis and are an important cause of morbidity and mortality. The diagnosis of pulmonary disease caused by NTM is hampered by its clinical similarity with tuberculosis (TB) and by the lack of an accurate and rapid laboratory diagnosis. OBJECTIVES Detect DNA from NTMs directly from lung samples using real-time polymerase chain reaction (qPCR) for amplification of 16S rRNA. Additionally, DNA sequencing (hsp65 and rpoB genes) was used to identify the species of MNTs. METHODS A total of 68 sputum samples (54 with suspected NTMs and 14 with TB) from patients treated at a referral hospital were used. FINDINGS Of these, 27/54 (50%) were qPCR positive for NTMs and 14/14 TB patients (controls) were qPCR negative with an almost perfect concordance (Kappa of 0.93) with the Mycobacterium spp. culture. Sequencing confirmed the presence of NTM in all positive samples. The most common species was Mycobacterium gordonae (33%), followed by Mycobacterium abscessus (26%), Mycobacterium fortuitum (22%), Mycobacterium avium (15%) and Mycobacterium peregrinum (4%). MAIN CONCLUSIONS The qPCR technique for detecting NTMs targeting 16S rRNA has the potential to detect NTMs and rapidly differentiate from Mycobacterium tuberculosis. However, it is necessary to identify the species to help in the differential diagnosis between disease and contamination, and to guide the choice of the therapeutic scheme |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Diagnóstico, Micobacterias no tuberculosas, Técnicas de diagnóstico, Reacción en cadena de la polimerasa (PCR) |
| Keyword: | Microbiology, Diagnosis, Non-tuberculous mycobacteria, Diagnostic techniques, Polymerase chain reaction (PCR) |
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