| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000446470 |
| ISSN: | 0074-0276 |
| Authors: | Goncalves, Cássia Cristina Alves1 Barroso, Shana Priscila Coutinho2 Herlinger, Alice Laschuk1 Galliez, Rafael de Mello3 Almeida, Tailah Bernardo de4 Boullosa, Lidia Theodoro1 Nascimento, Erica Ramos dos Santos1 Almeida, Jessica M. de1 Costa, Raissa Mirella dos Santos Cunha da1 Paixão, Tatiana Monteiro da5 Couceiro, José Nelson dos Santos Silva6 Frauches, Thiago Silva7 Souza-Junior, Wilson Rodrigues de7 Costa, Andréa Ribeiro7 Faffe, Débora Souza8 Leitão, Isabela de Carvalho8 Silva, Bianca Ortiz da9 Lira, Guilherme Sant’Anna de3 Almeida, Isabela Labarba Carvalho de3 Ferreira-Junior, Orlando da Costa1 Castiñeiras, Terezinha Marta Pereira Pinto3 Mariani, Diana1 Tanuri, Amilcar1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Instituto de Biologia, Rio de Janeiro. Brasil 2Hospital Naval Marcilio Dias, Instituto de Pesquisas Biomedicas, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Faculdade de Medicina, Rio de Janeiro. Brasil 4Instituto de Estudos do Mar Almirante Paulo Moreira, Departamento de Biotecnologia Marinha, Arraial do Cabo, Rio de Janeiro. Brasil 5Hospital Naval Marcilio Dias, Divisao de Doencas Infecto-Parasitarias, Rio de Janeiro. Brasil 6Universidade Federal do Rio de Janeiro, Instituto de Microbiologia "Paulo de Goes", Rio de Janeiro. Brasil 7Laboratorios Centrais de Saude Publica, Laboratorio Central Dr. Francisco Rimolo Neto, Marica, Rio de Janeiro. Brasil 8Universidade Federal do Rio de Janeiro, Instituto de Biofisica, Rio de Janeiro. Brasil 9Universidade Federal do Rio de Janeiro, Centro de Ciencias da Saude, Rio de Janeiro. Brasil 10Universidade Federal do Rio de Janeiro, Rio de Janeiro. Brasil |
| Year: | 2021 |
| Volumen: | 116 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The high demand for adequate material for the gold standard reverse transcription real-time polymerase chain reaction (RT-qPCR)-based diagnosis imposed by the Coronavirus disease 2019 (COVID-19) pandemic, combined with the inherent contamination risks for healthcare workers during nasopharyngeal swab (NP) sample collection and the discomfort it causes patients, brought the need to identify alternative specimens suitable for the diagnosis of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The aim of this work was to compare saliva and gingival fluid swabs to NP swabs as specimens for RT-qPCR-based SARS-CoV-2 diagnosis. We compared gingival fluid swabs (n = 158) and saliva (n = 207) to the rayon-tipped NP swabs obtained from mild-symptomatic and asymptomatic subjects as specimens for RT-qPCR for SARS-CoV-2 detection. When compared to NP swabs, gingival fluid swabs had a concordance rate of 15.4% among positive samples, zero among inconclusive, and 100% among negative ones. For saliva samples, the concordance rate was 67.6% among positive samples, 42.9% among inconclusive, and 96.8% among negative ones. However, the concordance rate between saliva and NP swabs was higher (96.9%) within samples with lower cycle threshold (Ct) values (Ct > 10 ≤ 25). Our data suggests that whereas gingival fluid swabs are not substitutes for NP swabs, saliva might be considered whenever NP swabs are not available or recommended |
| Disciplines: | Medicina |
| Keyword: | Virus, Diagnóstico, SARS-CoV-2, COVID-19, Técnicas de diagnóstico, Reacción en cadena de la polimerasa (PCR), Saliva, Fluido gingival |
| Keyword: | Virus, Diagnosis, SARS-CoV-2, COVID-19, Diagnostic techniques, Polymerase chain reaction (PCR), Saliva, Gingival fluid |
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