| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000352916 |
| ISSN: | 0074-0276 |
| Authors: | Gomes, Andreia Bartachini1 Soares, Killarney Ataide2 Bueno, Edneia Casagrande3 Espindola, Noeli Maria1 Iha, Alberto Hiroshi1 Maia, Antonio Augusto Mendes2 Peralta, Regina Helena Saramargo4 Vaz, Adelaide Jose1 |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Zootecnia e Engenharia de Alimentos, Pirassununga, Sao Paulo. Brasil 3Universidade do Vale do Itajai, Nucleo de Investigacoes Quimico-Farmaceuticas, Itajai, Santa Catarina. Brasil 4Universidade Federal Fluminense, Hospital Universitario Antonio Pedro, Niteroi, Rio de Janeiro. Brasil |
| Year: | 2007 |
| Season: | Sep |
| Volumen: | 102 |
| Number: | 6 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Seven swine were experimentally infected with Taenia solium eggs and blood samples from each animal were periodically collected. At the end of the experiment (t140) the animals did not show clinical aspects of cysticercosis or parasites in tongue inspection. All animals were slaughtered and cut into thin slices in searching for cysts. The number of cysts found in each animal varied from 1 to 85. Enzyme-linked immunosorbent assay (ELISA) tests for antibody (Ab) detection and for antigen (Ag) detection were performed, which presented respectively 71 and 57% of positivity. By immunoblot (IB), using 18/14(T. crassiceps Ag) or lentil-lectin-purified glycoproteins from T. solium Ag (LLGP) as Ag, five (71%) and six (86%) animals were positive, respectively. The association between Ag-ELISA with any IB (18/14 or LLGP) allowed the detection of all animals at 140 days post-experimental infection (days p.e.i.). The use of IB 18/14 combined to the Ag-ELISA allowed the detection of all animals since 70 days p.e.i., and the association between IB LLGP and Ag-ELISA allowed the detection of all animals since 112 days p.e.i. While all animals could be considered healthy by conventional screening tests, the use of immunoassays for detecting Ab and Ag showed better accuracy; therefore it would be more useful than usual clinical examination for screening cysticercosis in slightly infected pigs |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Porcinos, Parasitología, Cisticercosis porcina, Anticuerpos, Pruebas diagnósticas, Inmunoensayo, Taenia solium |
| Keyword: | Veterinary medicine and animal husbandry, Swine, Parasitology, Swine cysticercosis, Antibodies, Diagnostic tests, Immunoassay, Taenia solium |
| Full text: | Texto completo (Ver HTML) |
