| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000446472 |
| ISSN: | 0074-0276 |
| Authors: | Pérez, Cindy1 Díaz Roa, Andrea2 Bernal, Yuly3 Arenas, Nelson E3 Kalume, Dario Eluan4 Côrtes, Luzia Monteiro de Castro4 Silva-Junior, Pedro I. da2 Varela, Yahson5 Patarroyo, Manuel A5 Torres, Orlando1 Bello, Felio J6 |
| Institutions: | 1Universidad Antonio Nariño, Facultad de Medicina Veterinaria, Bogotá. Colombia 2Instituto Butantan, Laboratorio de Toxinología Aplicada, Sao Paulo. Brasil 3Universidad Antonio Nariño, Facultad de Ciencias, Bogotá. Colombia 4Fundacao Oswaldo Cruz, Instituto Oswaldo Cruz, Rio de Janeiro. Brasil 5Fundación Instituto de Inmunología de Colombia, Departamento de Biología Molecular e Inmunología, Bogotá. Colombia 6Universidad de la Salle, Facultad de Ciencias Agropecuarias, Bogotá. Colombia |
| Year: | 2021 |
| Volumen: | 116 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | The inappropriate use of antibiotics has led to the accelerated growth of resistance to antibiotics. The search for new therapeutic strategies (i.e., antimicrobial peptides-AMPs) has thus become a pressing need. OBJECTIVE Characterising and evaluating Sarconesiopsis magellanica larval fat body-derived AMPs. METHODS Fat body extracts were analysed by reversed-phase high-performance liquid chromatography (RP-HPLC); mass spectrometry was used for characterising the primary structure of the AMPs so found. ProtParam (Expasy) was used for analysing the AMPs’ physico-chemical properties. Synthetic AMPs’ antibacterial activity was evaluated. FINDINGS Four new AMPs were obtained and called sarconesin III, IV, V and VI. Sarconesin III had an α-helix structure and sarconesins IV, V and VI had linear formations. Oligomer prediction highlighted peptide-peptide interactions, suggesting that sarconesins III, V and VI could form self-aggregations when in contact with the microbial membrane. AMPs synthesised from their native molecules’ sequences had potent activity against Gram-positive bacteria and, to a lesser extent, against Gram-negative and drug-resistant bacteria. Sarconesin VI was the most efficient AMP. None of the four synthetic AMPs had a cytotoxic effect. MAIN CONCLUSIONS S. magellanica larval fat body-derived antimicrobial peptides are an important source of AMPs and could be used in different antimicrobial therapies and overcoming bacterial resistance |
| Disciplines: | Medicina |
| Keyword: | Química farmacéutica, Bioquímica, Péptidos antimicrobianos, Cuerpos grasos, Larvas, Sarconesiopsis magellanica, Diptera, Caracterización fisicoquímica, Actividad antibacteriana |
| Keyword: | Medicinal chemistry, Biochemistry, Antimicrobial peptides, Fat bodies, Larvae, Sarconesiopsis magellanica, Diptera, Physicochemical characterization, Antibacterial activity |
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