| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000452405 |
| ISSN: | 0074-0276 |
| Authors: | Silveira, Eduardo Lani Volpe da1 Rai, Urvashi1 Bonezi, Vivian2 Zárate Bladés, Carlos Rodrigo3 Claser, Carla4 |
| Institutions: | 1New York University, School of Medicine, Nueva York. Estados Unidos de América 2Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil 3Universidade Federal de Santa Catarina, Departamento de Microbiologia, Florianopolis, Santa Catarina. Brasil 4Universidade de Sao Paulo, Instituto de Ciencias Biomedicas, Sao Paulo. Brasil |
| Year: | 2022 |
| Volumen: | 117 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND It has been demonstrated that proteins expressed by liver-stage Plasmodium parasites can inhibit the translocation of transcription factors to the nucleus of different cells. This process would hinder the expression of immune genes, such as the CCL20 chemokine. OBJECTIVE Since CCR6 is the only cognate receptor for CCL20, we investigated the importance of this chemokine-receptor axis against rodent malaria. METHODS CCR6-deficient (KO) and wild-type (WT) C57BL/6 mice were challenged with Plasmodium berghei (Pb) NK65 sporozoites or infected red blood cells (iRBCs). Liver parasitic cDNA, parasitemia and serum cytokine concentrations were respectively evaluated through reverse transcription-polymerase chain reaction (RT-PCR), staining thin-blood smears with Giemsa solution, and enzyme-linked immunosorbent assay (ELISA). FINDINGS Although the sporozoite challenges yielded similar liver parasitic cDNA and parasitemia, KO mice presented a prolonged survival than WT mice. After iRBC challenges, KO mice kept displaying higher survival rates as well as a decreased IL-12 p70 concentration in the serum than WT mice. CONCLUSION Our data suggest that malaria triggered by PbNK65 liver- or blood-stage forms elicit a pro-inflammatory environment that culminates with a decreased survival of infected C57BL/6 mice |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Parasitología, Paludismo, Esporozoitos, Mecanismos de infección, Quimocinas, Plasmodium berghei |
| Keyword: | Microbiology, Parasitology, Malaria, Sporozoites, Infection mechanisms, Chemokines, Plasmodium berghei |
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