| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000413855 |
| ISSN: | 0074-0276 |
| Authors: | Chacha, Silvana Gama Florencio1 Gomes-Gouvea, Michele Soares3 Malta, Fernanda de Mello3 Ferreira, Sandro da Costa2 Villanova, Marcia Guimaraes2 Souza, Fernanda Fernandes2 Teixeira, Andreza Correa2 Passos, Afonso Dinis da Costa2 Pinho, Joao Renato Rebello3 Martinelli, Ana de Lourdes Candolo2 |
| Institutions: | 1Universidade Federal de Sao Carlos, Departamento de Medicina, Sao Carlos, Sao Paulo. Brasil 2Universidade de Sao Paulo, Faculdade de Medicina de Ribeirao Preto, Ribeirao Preto, Sao Paulo. Brasil 3Universidade de Sao Paulo, Instituto de Medicina Tropical, Sao Paulo. Brasil |
| Year: | 2017 |
| Season: | Sep |
| Volumen: | 112 |
| Number: | 9 |
| Pages: | 626-631 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | In Brazil, few studies have investigated the prevalence of infection with the precore (PC) and basal core promoter (BCP) mutants of the hepatitis B virus (HBV). OBJECTIVES This study aimed to analyse the frequency of PC and BCP mutations among patients infected with HBV and to evaluate the association between the variants and advanced hepatic disease. METHODS A total of 161 patients infected with HBV were studied. To identify PC and BCP mutations, a 501-bp fragment of HBV DNA was amplified and sequenced. FINDINGS PC and BCP regions from HBV strains were successfully amplified and sequenced in 129 and 118 cases, respectively. PC and BCP mutations were detected in 61.0% and 80.6% of the cases, respectively. The A1762T/G1764A variant was identified in 36.7% of the patients with grade 1 and 2 liver fibrosis (29/79) and in 81.8% of the patients with grade 3 and 4 liver fibrosis (9/11) (p < 0.01); in 76.9% of the patients with cirrhosis (10/13) and in 38.1% of the patients without cirrhosis (40/105) (p = 0.01); and in 77.8% of the patients with hepatocellular carcinoma (HCC) (7/9) and in 39.4% of the patients without HCC (43/109) (p = 0.03). MAIN CONCLUSIONS A high prevalence of HBV PC and BCP mutants was found. The A1762T/G1764A variant was independently associated with advanced forms of liver fibrosis, hepatic cirrhosis, and HCC |
| Disciplines: | Medicina |
| Keyword: | Virus, Genética, Virus de la hepatitis B, Mutaciones, Virulencia, Prevalencia |
| Keyword: | Medicine, Virus, Genetics, Hepatitis B virus, Mutations, Virulence, Prevalence |
| Full text: | Texto completo (Ver HTML) |
