| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000391619 |
| ISSN: | 0074-0276 |
| Authors: | Braga, Andre Flores1 Moretto, Daniela Ferraz2 Gigliotti, Patrícia1 Peruchi, Mariela3 Vilani-Moreno, Fatima Regina4 Campanelli, Ana Paula5 Latini, Ana Carla Pereira4 Iyer, Anand6 Das, Pranab Kumar4 Souza, Vania Nieto Brito de4 |
| Institutions: | 1University of Amsterdam, Academic Medical Center, Amsterdam. Países Bajos |
| Year: | 2015 |
| Season: | Ago |
| Volumen: | 110 |
| Number: | 5 |
| Pages: | 655-661 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Dendritic cells (DCs) play a pivotal role in the connection of innate and adaptive immunity of hosts to mycobacterial infection. Studies on the interaction of monocyte-derived DCs (MO-DCs) using Mycobacterium leprae in leprosy patients are rare. The present study demonstrated that the differentiation of MOs to DCs was similar in all forms of leprosy compared to normal healthy individuals. In vitro stimulation of immature MO-DCs with sonicated M. leprae induced variable degrees of DC maturation as determined by the increased expression of HLA-DR, CD40, CD80 and CD86, but not CD83, in all studied groups. The production of different cytokines by the MO-DCs appeared similar in all of the studied groups under similar conditions. However, the production of interleukin (IL)-12p70 by MO-DCs from lepromatous (LL) leprosy patients after in vitro stimulation with M. leprae was lower than tuberculoid leprosy patients and healthy individuals, even after CD40 ligation with CD40 ligand-transfected cells. The present cumulative findings suggest that the MO-DCs of LL patients are generally a weak producer of IL-12p70 despite the moderate activating properties ofM. leprae. These results may explain the poor M. leprae-specific cell-mediated immunity in the LL type of leprosy |
| Disciplines: | Medicina |
| Keyword: | Dermatología, Neurología, Inmunología, Lepra, Respuesta inmune, Células dendríticas, Citocinas, Mycobacterium leprae |
| Keyword: | Medicine, Dermatology, Neurology, Immunology, Leprosy, Immune response, Dendritic cells, Cytokines, Mycobacterium leprae |
| Full text: | Texto completo (Ver HTML) |
