Optimization of extraction conditions for flavonoids of Physalis alkekengi var. franchetii stems by response surface methodology and inhibition of acetylcholinesterase activity
Document title: Optimization of extraction conditions for flavonoids of Physalis alkekengi var. franchetii stems by response surface methodology and inhibition of acetylcholinesterase activity
Journal: Journal of the Mexican Chemical Society
Database: PERIÓDICA
System number: 000384157
ISSN: 1665-9686
Authors: 1
1
1
2
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2
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Institutions: 1Shenyang University of Chemical Technology, College of Pharmaceutical and Biological Engineering, Shenyang, Liaoning. China
2Shenyang University of Chemical Technology, College of Chemical Engineering, Shenyang, Liaoning. China
Year:
Season: Ene-Mar
Volumen: 59
Number: 1
Country: México
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
Spanish abstract En esta investigación se optimizaron las condiciones de extracción de flavonoides a partir de Physalis alkekengi var. Franchetii, asistida por microondas, usando un diseño L33 Box-Behenke. Las condiciones determinadas fueron etanol 60%, la relación líquido:sólido fue de 12.4 y se usaron 531.4 W para el microondas. El rendimiento obtenido fue de 3.85 mg g-1, el cual es cercano al valor predicho. El extracto obtenido inhibe de forma importante la actividad de la acetilcoliestearasa, con una IC50 de 11.61 µg mL-1 y una inhibición máxima de 89.91%. Estos resultados son mejores que los obtenidos con el compuesto obtenido mediante extracción por reflujo
English abstract The microwave-assisted extraction conditions of flavonoids in Physalis alkekengi. var. franchetii stems were optimized using a L33 Box-Behnken Design. The optimized extraction conditions were determined as follows: 60% for the ethanol concentration, 12.4 for the liquid-to-solid ratio, and 531.4 W for the microwave power, respectively. The yield of the extract obtained under the optimized conditions was 3.85 mg g-1 which was close to the predicted value. In addition, the extract exhibited potent acetylcholinesterase inhibitory activity, with the IC50 value of 11.61 μg mL-1 and a maximal inhibition ratio 89.81%. Both the yield and activity were better than reflux extraction
Disciplines: Biología,
Química
Keyword: Angiospermas,
Fitoquímica,
Química farmacéutica,
Physalis alkekengi,
Flavonoides,
Acetilcolinesterasa,
Microondas
Keyword: Biology,
Chemistry,
Angiosperms,
Medicinal chemistry,
Phytochemistry,
Physalis alkekengi,
Flavonoids,
Acetylcholinesterase,
Microwave
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