| Journal: | Jornal de pediatria |
| Database: | PERIÓDICA |
| System number: | 000298291 |
| ISSN: | 0021-7557 |
| Authors: | Souza, Helena A.P.H.M1 Nogueira, Keite S Matos, Adriana P Vieira, Ricardo P1 Riedi, Carlos A Rosario, Nelson A Telles, Flavio Q Dalla Costa, Libera M |
| Institutions: | 1Universidade Federal do Parana, Curitiba, Parana. Brasil |
| Year: | 2006 |
| Season: | Sep-Oct |
| Volumen: | 82 |
| Number: | 5 |
| Pages: | 377-382 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Aplicado |
| English abstract | OBJECTIVES: To assess bacterial colonization prospectively in patients with cystic fibrosis identified by neonatal screening. To assess susceptibility to antimicrobials and to perform the molecular typing of Staphylococcus aureus strains isolated from the oropharynx of patients during the study. METHODS: Twenty-five cystic fibrosis patients receiving regular treatment at the Cystic Fibrosis Outpatient Clinic of Hospital de Clínicas of Universidade Federal do Paraná Brazil, were included in the study. All patients were identified by trypsin-like immunoreactivity and their diagnosis was confirmed by two or more sweat tests. Oropharyngeal swabs were collected and cultured according to routine methods; bacterial colonies were phenotypically identified and their susceptibility to antimicrobials was tested. S. aureus isolates were submitted to molecular typing using pulsed-field gel electrophoresis. RESULTS: Out of 234 oropharyngeal swabs, S. aureus was the most frequently isolated strain (76% of patients, 42% of swabs), followed by Pseudomonas aeruginosa (36% of patients, 16% of swabs) and Haemophilus spp. (76% of patients; 19% of swabs). Seventy-three isolates were obtained from 19 patients colonized with S. aureus, of which 18 were oxacillin-resistant (24.6%), isolated from two patients, with the same electrophoretic profiles as that of the Brazilian clone. The remaining oxacillin-sensitive isolates were distributed into 18 electrophoretic profiles. CONCLUSION: There was higher prevalence of S. aureus, with earlier isolation than other pathogens. Multi-sensitive isolates were distributed into different clones, characterizing non-transmissibility among community-acquired strains. The isolated oxacillin-resistant S. aureus showed identical electrophoretic profiles, probably acquired in hospital. P. aeruginosa was not so frequent in the studied population |
| Portuguese abstract | OBJETIVOS: Avaliar prospectivamente a colonização bacteriana de pacientes com fibrose cística identificados por triagem neonatal. Avaliar a suscetibilidade a antimicrobianos e caracterizar molecularmente as cepas de Staphylococcus aureus isoladas da orofaringe dos pacientes no período do estudo. MÉTODOS: Foram estudados 25 pacientes com fibrose cística, identificados por tripsina imunorreativa e com diagnóstico confirmado por duas ou mais provas de suor, atendidos regularmente no ambulatório de fibrose cística do Hospital de Clínicas da Universidade Federal do Paraná. Foram coletadas amostras de orofaringe com swab e cultivadas por métodos rotineiros; as colônias bacterianas foram identificadas fenotipicamente e testadas quanto à suscetibilidade a antimicrobianos. Os isolados de S. aureus foram submetidos a tipagem molecular por eletroforese em campo pulsado. RESULTADOS: De um total de 234 amostras de orofaringe, S. aureus foi isolado em maior número (76% dos pacientes, 42% das amostras), seguido de Pseudomonas aeruginosa (36% dos pacientes, 16% das amostras) e Haemophilus spp. (76% dos pacientes; 19% das amostras). Dos 19 pacientes colonizados com S. aureus, foram obtidos 73 isolados, 18 oxacilina-resistentes (24,6%), isolados de dois pacientes, com perfis eletroforéticos idênticos ao do clone brasileiro. Os demais isolados oxacilina-sensíveis distribuíram-se entre 18 perfis eletroforéticos distintos. CONCLUSÃO: Observou-se uma maior prevalência de S. aureus, com isolamento mais precoce em relação aos outros patógenos pesquisados |
| Disciplines: | Medicina |
| Keyword: | Microbiología, Pediatría, Genética, Fibrosis quística, Colonización bacteriana, Staphylococcus aureus, Epidemiología, Tamizaje neonatal |
| Keyword: | Medicine, Microbiology, Pediatrics, Genetics, Cystic fibrosis, Bacterial settlement, Staphylococcus aureus, Epidemiology, Neonatal screening |
| Full text: | Texto completo (Ver HTML) |
