| Journal: | Jornal brasileiro de pneumologia |
| Database: | PERIÓDICA |
| System number: | 000297390 |
| ISSN: | 1806-3713 |
| Authors: | Rodrigues-Junior, Jose Maciel1 Lima, Karla de Melo Castelo, Arlete Aparecida Martins Coelho Martins, Vania Luiza Deperon Bonato Santos, Sandra Aparecida dos Faccioli, Lucia Helena Silva, Celio Lopes |
| Institutions: | 1Universidade de Sao Paulo, Faculdade de Medicina, Ribeirao Preto, Sao Paulo. Brasil |
| Year: | 2004 |
| Season: | Jul-Ago |
| Volumen: | 30 |
| Number: | 4 |
| Pages: | 378-387 |
| Country: | Brasil |
| Language: | Portugués |
| Document type: | Artículo |
| Approach: | Teórico, descriptivo |
| English abstract | The DNA vaccines currently under pre-clinical and clinical development may prove to be important tools in combating infectious diseases, such as tuberculosis, for which no safe and effective form of prevention has yet been developed. In recent years, several studies have aimed to develop a DNA vaccine encoding mycobacterial proteins such as antigen 85 (Ag85) and the 65-kDa mycobacterial heat shock protein (hsp65). The latter is protective against virulent infection with Mycobacterium tuberculosis (including multidrug-resistant strains). The hsp65 DNA vaccine, currently under clinical evaluation in Brazil for cancer therapy, is able to induce the secretion of Th1 cytokines, such as gamma-interferon, associated with disease control. Furthermore, this vaccine stimulates cytotoxic CD8 and CD4 T-cell clones that can be characterized as memory cells, which are responsible for effective and long-lasting immunity against tuberculosis. When used as a therapeutic agent in inoculated mice, the hsp65 DNA vaccine promotes changes in the immunity profile, triggering the secretion of Th1 cytokines and establishing a favorable environment for the elimination of bacilli. The results also demonstrate that the route of administration, as well as the formulation in which the vaccine is administered, fundamentally influence the pattern and duration of the immune response induced. Taking all currently available data into account, we can conclude that a DNA vaccine against tuberculosis could contribute significantly to the control of the disease |
| Portuguese abstract | Quando utilizada na terapia da infecção, a vacina de DNA-hsp65 faz com que haja uma mudança no padrão de resposta imune, induzindo a secreção de citocinas de padrão Th1 criando um ambiente favorável à erradicação do bacilo. Os resultados demonstram ainda que a via de administração e a formulação na qual a vacina é administrada exerce fundamental influência no padrão e duração da resposta imune desencadeada. O conjunto de resultados hoje disponíveis mostra que uma vacina de DNA contra a tuberculose contribuirá de maneira significativa no controle desta doença |
| Disciplines: | Medicina |
| Keyword: | Neumología, Genética, Inmunología, Tuberculosis, Vacunas, ADN, Choque térmico, Proteínas, Autoinmunidad |
| Keyword: | Medicine, Pneumology, Tuberculosis, Vaccines, DNA, Thermal shock, Proteins, Autoimmunity, Genetics, Immunology |
| Full text: | Texto completo (Ver HTML) |
