| Journal: | International journal of morphology |
| Database: | PERIÓDICA |
| System number: | 000268069 |
| ISSN: | 0717-9502 |
| Authors: | Schencke, Carolina del1 Sol, Mariano del2 Rojas, Mariana3 |
| Institutions: | 1Universidad de La Frontera, Temuco, Cautín. Chile 2Universidad de La Frontera, Facultad de Medicina, Temuco, Cautín. Chile 3Universidad de Chile, Facultad de Medicina, Santiago de Chile. Chile |
| Year: | 2005 |
| Season: | Dic |
| Volumen: | 22 |
| Number: | 4 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Descriptivo |
| Spanish abstract | La primera adhesión, ocurre entre el sinciciotrofoblasto y las células epiteliales de la pared antimesometrial antes de que ocurra una modificación epitelial general y del tejido conjuntivo subyacente, con aumento de glicógeno y glicosaminoglicanos |
| English abstract | Embryo implantation in mammalian uterus starts by a cell-to-cell direct contact between the blastocyst's trophoblast and the uterine ephitelium. The rabbit is a good model to study implantation. This work aims at analyzing the sequence of morphological morphometric events during implantation (7 to 10 days post-coitum). Sixteen white New Zealand adult female rabbits (Oryctolagus cuniculus), from the Faculty of Medicine, University of La Frontera, Chile, were used. After sacrifice, the uterus of each pregnant female was fixed in 10% formaline and postfixed in Duboscq Brasil. Stainings used were HE and Masson Trichromic and for histochemistry PAS, Diastase-PAS, Alcian Blue (pH 1 and 2.5) and Junqueira Picrosirius. Some samples were fixed in Methacarn for immunocitochemical study using the monoclonal AE1 antibody to reveal changes in the intermediate filaments in the ephitelial cells. Diameter of the embryonic vesicle in mm and uterine glands in µm were recorded. From day 7 to 10 post coitum, the diameter of the embryonic vesicle increased 2mm per day. The uterine glands underwent significative growth, differentially depending if they were located in the mesometrial or antimesometrial uterine wall. In the rabbit, the syncitiotrophoblast of the embryonic hemisphere of the blastocyst adhieres and fuses to the uterine ephitelium. The implantational chamber in the rabbit, is therefore formed as a result of the expansion of the blastocysts which contacts with many points of the uterine wall, either in the mesometrial or antimesometrial uterine wall. The first adhesion occurs between the syntitiotrophoblast and the ephitelial cells of the antimesometrial wall prior to a general ephitelial modification and the subjacent connective tissue, which shows increased glycogen and glycosamineglycanes |
| Disciplines: | Medicina |
| Keyword: | Medicina experimental, Animales de laboratorio, Embriología, Utero, Morfología, Conejos, Embriones |
| Keyword: | Medicine, Experimental medicine, Laboratory animals, Uterus, Embryos, Morphology |
| Full text: | Texto completo (Ver HTML) |
