| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000346447 |
| ISSN: | 1415-4757 |
| Authors: | Sa, Maria Eugenia Lisei de1 Lopes, Marcus Jose Conceicao2 Campos, Magnolia de Araujo2 Paiva, Luciano Vilela3 Santos, Regina Maria Amorim dos5 Beneventi, Magda Aparecida5 Firmino, Alexandre Augusto Pereira4 Sa, Maria Fatima Grossi de5 |
| Institutions: | 1Empresa de Pesquisa Agropecuaria de Minas Gerais, Uberaba, Minas Gerais. Brasil 2Universidade Federal de Campina Grande, Centro de Educacao e Saude, Cuite, Paraiba. Brasil 3Universidade Federal de Lavras, Lavras, Minas Gerais. Brasil 4Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul. Brasil 5Empresa Brasileira de Pesquisa Agropecuaria, Recursos Geneticos e Biotecnologia, Brasilia, Distrito Federal. Brasil |
| Year: | 2012 |
| Season: | May |
| Volumen: | 35 |
| Pages: | 272-282 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Soybean is an important crop for Brazilian agribusiness. However, many factors can limit its production, especially root-knot nematode infection. Studies on the mechanisms employed by the resistant soybean genotypes to prevent infection by these nematodes are of great interest for breeders. For these reasons, the aim of this work is to characterize the transcriptome of soybean line PI 595099-Meloidogyne javanica interaction through expression analysis. Two cDNA libraries were obtained using a pool of RNA from PI 595099 uninfected and M. javanica (J2) infected roots, collected at 6, 12, 24, 48, 96, 144 and 192 h after inoculation. Around 800 ESTs (Expressed Sequence Tags) were sequenced and clustered into 195 clusters. In silico subtraction analysis identified eleven differentially expressed genes encoding putative proteins sharing amino acid sequence similarities by using BlastX: metallothionein, SLAH4 (SLAC1 Homologue 4), SLAH1 (SLAC1 Homologue 1), zinc-finger proteins, AN1-type proteins, auxin-repressed proteins, thioredoxin and nuclear transport factor 2 (NTF-2). Other genes were also found exclusively in nematode stressed soybean roots, such as NAC domain-containing proteins, MADS-box proteins, SOC1 (suppressor of overexpression of constans 1) proteins, thioredoxin-like protein 4-Coumarate-CoA ligase and the transcription factor (TF) MYBZ2. Among the genes identified in non-stressed roots only were Ser/Thr protein kinases, wound-induced basic protein, ethylene-responsive family protein, metallothionein-like protein cysteine proteinase inhibitor (cystatin) and Putative Kunitz trypsin protease inhibitor. An understanding of the roles of these differentially expressed genes will provide insights into the resistance mechanisms and candidate genes involved in soybean-M. javanica interaction and contribute to more effective control of this pathogen |
| Disciplines: | Agrociencias |
| Keyword: | Fitotecnia, Genética, Mejoramiento genético, Resistencia a plagas, Fitoparásitos, Expresión génica, Transcriptoma, Meloidogyne javanica, Soya, Glycine max, Leguminosae |
| Keyword: | Agricultural sciences, Crop husbandry, Genetics, Plant breeding, Phytoparasites, Gene expression, Pests resistance, Transcriptome, Meloidogyne javanica, Soybean, Glycine max, Leguminosae |
| Full text: | Texto completo (Ver HTML) |
