| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000408464 |
| ISSN: | 1415-4757 |
| Authors: | Rodrigues, Nureyev F1 Fonseca, Guilherme C. da2 Kulcheski, Franceli R2 Margis, Rogerio1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Departamento de Genetica, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul. Brasil |
| Year: | 2017 |
| Volumen: | 40 |
| Pages: | 200-208 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated libraries. RT-qPCR assays demonstrated an increase in editing efficiency of selected genes. The salt stress enhanced the RNA editing process in transcripts, indicating responses to components of the electron transfer chain, photosystem and translation complexes. These increases can be a response to keep the homeostasis of chloroplast protein functions in response to salt stress |
| Disciplines: | Biología |
| Keyword: | Botánica, Fisiología vegetal, Genética, Biología molecular, Soya, ARN mitocondrial, Cloroplastos, Estrés salino, Glycine max |
| Keyword: | Biology, Botany, Genetics, Plant physiology, Molecular biology, Soybean, Mitochondrial RNA, Chloroplasts, Saline stress, Glycine max |
| Full text: | Texto completo (Ver HTML) |
