| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000420046 |
| ISSN: | 1415-4757 |
| Authors: | Rincão, Michelle Pires1 Carvalho, Mayra Costa da Cruz Gallo de3 Nascimento, Leandro Costa4 Lopes-Caitar, Valéria S2 Carvalho, Kenia de2 Darben, Luana M2 Yokoyama, Alessandra5 Carazzolle, Marcelo Falsarella4 Abdelnoor, Ricardo Vilela2 Marcelino-Guimarães, Francismar Correa2 |
| Institutions: | 1Universidade Estadual de Londrina, Programa de Pos-Graduacao em Genetica e Biologia Molecular, Londrina, Parana. Brasil 2Empresa Brasileira de Pesquisa Agropecuaria, Soja, Londrina, Parana. Brasil 3Universidade Estadual do Norte do Parana, Bandeirantes, Parana. Brasil 4Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil 5Universidade Estadual de Londrina, Programa de Pos-Graduacao em Biotecnologia, Londrina, Parana. Brasil |
| Year: | 2018 |
| Season: | Sep |
| Volumen: | 41 |
| Number: | 3 |
| Pages: | 671-691 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | Asian soybean rust (ASR) is one of the most destructive diseases affecting soybeans. The causative agent of ASR, the fungus Phakopsora pachyrhizi , presents characteristics that make it difficult to study in vitro, limiting our knowledge of plant-pathogen dynamics. Therefore, this work used leaf lesion laser microdissection associated with deep sequencing to determine the pathogen transcriptome during compatible and incompatible interactions with soybean. The 36,350 generated unisequences provided an overview of the main genes and biological pathways that were ac- tive in the fungus during the infection cycle. We also identified the most expressed transcripts, including sequences similar to other fungal virulence and signaling proteins. Enriched P. pachyrhizi transcripts in the resistant (PI561356) soybean genotype were related to extracellular matrix organization and metabolic signaling pathways and, among infection structures, in amino acid metabolism and intracellular transport. Unisequences were further grouped into gene families along predicted sequences from 15 other fungi and oomycetes, including rust fungi, allowing the identi- fication of conserved multigenic families, as well as being specific to P. pachyrhizi . The results revealed important biological processes observed in P. pachyrhizi , contributing with information related to fungal biology and, consequently, a better understanding of ASR |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Fitopatología, Hongos, Genética, Roya asiática de la soya, Phakopsora pachyrhizi, Familias multigénicas, Elementos transponibles |
| Keyword: | Phytopathology, Fungi, Genetics, Asian soybean rust, Phakopsora pachyrhizi, Multigenic families, Transposable elements |
| Full text: | Texto completo (Ver PDF) |
