| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459232 |
| ISSN: | 1415-4757 |
| Authors: | Liu, Weiwei1 Cheng, Jing2 |
| Institutions: | 1Maternal and Child Health Hospital of Hubei Province, Department of Gynecology, Wuhan, Hubei. China 2Renmin Hospital of Wuhan University, Department of Obstetrics and Gynecology, Wuhan, Hubei. China |
| Year: | 2022 |
| Volumen: | 45 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | The function and mechanism of long intergenic non-protein coding RNA 974 (LINC00974) are rarely reported in ovarian cancer (OC). The study aimed to investigate how LINC00974 affects the progression of OC. The expression levels of LINC00974, microRNA-33a (miR-33a), and high mobility group box 2 (HMGB2) mRNA were detected by qRT-PCR. The LINC00974/miR-33a/HMGB2 axis was confirmed by dual-luciferase reporter, RNA-binding protein immunoprecipitation (RIP), and biotinylated RNA pull-down assays. A series of in vitro experiments were employed to assess the effects of LINC00974/miR-33a/HMGB2 axis on the proliferation, invasion and epithelial mesenchymal transition (EMT) of OC cells. Results showed that LINC00974 and HMGB2 mRNA expression were upregulated in OC cells, while miR-33a expression was downregulated. HMGB2 was a direct target gene of miR-33a. LINC00974 act as a competing endogenous RNA (ceRNA) to directly bind with miR-33a, thereby upregulated HMGB2 expression. Notably, silencing of LINC00974 suppressed cell proliferation, invasion and EMT of OC cells, whereas miR-33a knockdown partially reversed the phenotypes of LINC00974 on OC cells. Overall, our study demonstrated that LINC00974 sponges miR-33a to promote cell proliferation, invasion, and EMT of OC through HMGB2 upregulation. LINC00974/miR-33a/HMGB2 axis may be an important signaling pathway in the progression of OC |
| Disciplines: | Biología, Medicina |
| Keyword: | Genética, Oncología, LINC00974, MiR-33a, HMGB2, Cáncer de ovarios |
| Keyword: | Genetics, Oncology, LINC00974, MiR-33a, HMGB2, Ovarian cancer |
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