| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000418554 |
| ISSN: | 1415-4757 |
| Authors: | Zepeta-Flores, Nahum1 Valverde, Mahara1 Lopez-Saavedra, Alejandro2 Rojas, Emilio1 |
| Institutions: | 1Universidad Nacional Autónoma de México, Departamento de Medicina Genómica y Toxicología Ambiental, Ciudad de México. México 2Universidad Nacional Autónoma de México, Instituto de Investigaciones Biomédicas, Ciudad de México. México |
| Year: | 2018 |
| Season: | Jun |
| Volumen: | 41 |
| Number: | 2 |
| Pages: | 475-487 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | The importance of glutathione (GSH) in alternative cellular roles to the canonically proposed, were analyzed in a model unable to synthesize GSH. Gene expression analysis shows that the regulation of the actin cytoskeleton pathway is strongly impacted by the absence of GSH. To test this hypothesis, we evaluate the effect of GSH depletion via buthionine sulfoximine (5 and 12.5 mM) in human neuroblastoma MSN cells. In the present study, 70% of GSH reduction did not induce reactive oxygen species, lipoperoxidation, or cytotoxicity, which enabled us to evaluate the effect of glutathione in the absence of oxidative stress. The cells with decreasing GSH levels acquired morphology changes that depended on the actin cytoskeleton and not on tubulin. We evaluated the expression of three actin-binding proteins: thymosin 4, profilin and gelsolin, showing a reduced expression, both at gene and protein levels at 24 hours of treatment; however, this suppression disappears after 48 hours of treatment. These changes were sufficient to trigger the co-localization of the three proteins towards cytoplasmic projections. Our data confirm that a decrease in GSH in the absence of oxidative stress can transiently inhibit the actin binding proteins and that this stimulus is sufficient to induce changes in cellular morphology via the actin cytoskeleton |
| Disciplines: | Química, Biología |
| Keyword: | Bioquímica, Biología celular, Genética, Glutatión, BSO, Timosina, Gelsolina, Perfilado |
| Keyword: | Biochemistry, Cell biology, Genetics, Glutathione, BSO, Thymosin 4, Gelsolin, Profiling |
| Full text: | Texto completo (Ver PDF) |
