| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459229 |
| ISSN: | 1415-4757 |
| Authors: | Comerlato, Carolina Baldisserotto1 Prichula, Janira2 Siqueira, Franciele Maboni3 Ritter, Ana Carolina1 Varela, Ana Paula Muterle4 Mayer, Fabiana Quoos4 Brandelli, Adriano1 |
| Institutions: | 1Universidade Federal do Rio Grande do Sul, Departamento de Ciencia de Alimentos, Porto Alegre, Rio Grande do Sul. Brasil 2Universidade Federal de Ciencias da Saude de Porto Alegre, Laboratorio de Cocos Gram-Positivos, Porto Alegre, Rio Grande do Sul. Brasil 3Universidade Federal do Rio Grande do Sul, Departamento de Patologia Clinica Veterinaria, Porto Alegre, Rio Grande do Sul. Brasil 4Secretaria da Agricultura Pecuaria e Irrigacao do Rio Grande do Sul, Departamento de Diagnostico e Pesquisa Agropecuaria, Eldorado do Sul, Rio Grande do Sul. Brasil |
| Year: | 2022 |
| Volumen: | 45 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Gut microbiota exerts a fundamental role in human health and increased evidence supports the beneficial role of probiotic microorganisms in the maintenance of intestinal health. Enterococcus durans LAB18S was previously isolated from soft cheese and showed some desirable in vitro probiotic properties, for that reason its genome was sequenced and evaluated for genes that can be relevant for probiotic activity and are involved in selenium metabolism. Genome sequencing was performed using the Illumina MiSeq System. A variety of genes potentially associated with probiotic properties, including adhesion capability, viability at low pH, bile salt resistance, antimicrobial activity, and utilization of prebiotic fructooligosaccharides (FOS) were identified. The strain showed tolerance to acid pH and bile salts, exhibited antimicrobial activity and thrived on prebiotic oligosaccharides. Six genes involved in selenium metabolism were predicted. Analysis of the SECIS element showed twelve known selenoprotein candidates. E. durans LAB18S was the only food isolate showing absence of plasmids, virulence and antimicrobial resistance genes, when compared with other 30 E. durans genomes. The results of this study provide evidence supporting the potential of E. durans LAB18S as alternative for probiotic formulations |
| Disciplines: | Biología, Química |
| Keyword: | Genética, Química de alimentos, Bacterias, Enterococcus durans, Probióticos, Prebióticos, Genoma, Selenoproteínas, Queso |
| Keyword: | Genetics, Food chemistry, Bacteria, Cheese, Enterococcus durans, Probiotics, Prebiotics, Genome, Selenoproteins |
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