| Revista: | Genetics and molecular biology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000337776 |
| ISSN: | 1415-4757 |
| Autores: | Fazza, Ana Cristina1 Sabino, Flavia Cal1 Setta, Nathalia de1 Bordin-Junior, Newton Antonio2 Silva, Eloiza Helena Tajara da3 Carareto, Claudia Marcia Aparecida1 |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Sao Jose do Rio Preto, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Medicina, Sao Jose do Rio Preto, Sao Paulo. Brasil 3Universidade de Sao Paulo, Instituto de Biociencias, Sao Paulo. Brasil |
| Año: | 2009 |
| Periodo: | Mar |
| Volumen: | 32 |
| Número: | 1 |
| Paginación: | 25-31 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Analítico, descriptivo |
| Resumen en inglés | Alu-PCR is a relatively simple technique that can be used to investigate genomic instability in cancer. This technique allows identification of the loss, gain or amplification of gene sequences based on the analysis of segments between two Alu elements coupled with quantitative and qualitative analyses of the profiles obtained from tumor samples, surgical margins and blood. In this work, we used Alu-PCR to identify gene alterations in ten patients with invasive ductal breast cancer. Several deletions and insertions were identified, indicating genomic instability in the tumor and adjacent normal tissue. Although not associated with specific genes, the alterations, which involved chromosomal bands 1p36.23, 1q41, 11q14.3, 13q14.2, occurred in areas of well-known genomic instability in breast and other types of cancer. These results indicate the potential usefulness of Alu-PCR in identifying altered gene sequences in breast cancer. However, caution is required in its application since the Alu primer can produce non-specific amplification |
| Disciplinas: | Química, Biología, Medicina |
| Palabras clave: | Bioquímica, Biología celular, Genética, Oncología, Cáncer, Mama, Mutaciones, Carcinoma, Recombinación |
| Keyword: | Chemistry, Biology, Medicine, Biochemistry, Cell biology, Genetics, Oncology, Cancer, Breast, Mutations, Carcinoma, Recombination |
| Texto completo: | Texto completo (Ver HTML) |
