| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459180 |
| ISSN: | 1415-4757 |
| Authors: | Aygün, Ilkin1 Rzepczak, Alicja1 Miki, Takashi S1 |
| Institutions: | 1Polish Academy of Sciences, Institute of Bioorganic Chemistry, Poznan. Polonia |
| Year: | 2023 |
| Season: | Ene |
| Volumen: | 46 |
| Number: | 2 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | XRN2 is an evolutionarily conserved 5’-to-3’ exoribonuclease, which degrades or trims various types of RNA in the nucleus. Although XRN-2 is essential for embryogenesis, larval development and reproduction in Caenorhabditis elegans, relevant molecular pathways remain unidentified. Here we create a germline-specific xrn-2 conditional mutant and perform a mutagenesis screen for suppressors of sterility. Loss-of-function alleles of dpy-10, osr-1, ptr-6 and C34C12.2 genes are identified. Depletion of DPY-10, OSR-1 or PTR-6 increases expression of gpdh-1 that encodes a glycerol-3-phosphate dehydrogenase, thereby elevates glycerol accumulation to suppress sterility of the mutant. The C34C12.2 protein is predominantly localized in the nucleolus of germ cells and shows a similarity to Saccharomyces cerevisiae Net1, which is involved in rDNA silencing. Depletion of NRDE-2, a putative interacting partner of C34C12.2 and a component of the nuclear RNAi machinery, restores fertility to the xrn-2 conditional mutant. These results may help to identify an essential role of XRN-2 in germline development |
| Disciplines: | Biología |
| Keyword: | Helmintos, Genética, Nematoda, Caenorhabditis elegans, Mutagénesis |
| Keyword: | Helminths, Genetics, Nematoda, Caenorhabditis elegans, Mutagenesis |
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