| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000398046 |
| ISSN: | 0717-3458 |
| Authors: | Ma, Yuan-Yuan2 Guo, Xiu-Lin1 Liu, Bin-Hui4 Liu, Zi-Hui1 Shao, Hong-Bo3 |
| Institutions: | 1Hebei Academy of Agricultural and Forestry Sciences, Institute of Genetics and Physiology, Shijiazhuang, Hebei. China 2Shijiazhuang University, School of Chemical Engineering, Shijiazhuang, Hebei. China 3Chinese Academy of Sciences, Yantai Institute of Coastal Zone Research, Yantái, Shandong. China 4Hebei Academy of Agricultural and Forestry Sciences, Institue of Dryland Farming, Hengshui. China |
| Year: | 2011 |
| Volumen: | 14 |
| Number: | 3 |
| Pages: | 1-10 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Analítico, descriptivo |
| English abstract | The changes of cell ultrastructure as well as Ca 2+ homeostasis involved in the drought - induced maize leaf senescence was investigated. Meanwhile, many indicatives of leaf senescence including thiobarbituric acid reactive substance (MDA), electrolyte leakage (EL), and chlorophyll along with soluble proteins were also detected during the process. The Polyethylene glycol6000(PEG6000) - incubated detached leaves showed a slight increase in the MDA content and electrolyte leakage during the first 30 min of our detection, which was corresponded to an unobvious alt eration of the cell ultrastructure. Other typical senescence parameters measured in whole leaf exhibited a moderate elevation as well. Thereafter, however, the EL and MDA rose to a large extent, which was correlated with a dramatic damage to the cell ultrastructure with concomitant sharp decrease in the chlorophyll and soluble proteins content. The deposits of calcium antimonite, being an indicator for Ca 2+ localization, were observed in the vacuoles as well as intercellular spaces in the leaves grown under normal condition. Nevertheless, after PEG treatment, it was revealed a distinct increment of Ca 2+ in the cyt oplasm as well as chloroplasts and nuclei. Moreover, with long-lasting treatment of PEG to the detached leaves, the concentration of Ca 2+ as descri bed above showed a continuous increment which was consist with the remarked alteration of physiological parameters and severe damage to the ultrastructure of cells, all of which indicated the leaf senescence. Such drought-induced leaf senescence might resu lt from a loss of the cell’s capability to extrude Ca 2+. All above findings give us a good insight into the important role of Ca 2+ homeostasis in the process of leaf senescence accelerated by the drought stress |
| Disciplines: | Biología |
| Keyword: | Biología celular, Fisiología vegetal, Bioquímica, Homeostasis, Calcio, Sequía, Senescencia foliar, Maíz, Ultraestructura, Organelos, Transducción de señales |
| Keyword: | Biology, Cell biology, Plant physiology, Biochemistry, Homeostasis, Calcium, Drought, Leaf senescence, Maize, Organelles, Ultrastructure, Signal transduction |
| Full text: | Texto completo (Ver PDF) |
