| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000357853 |
| ISSN: | 0717-3458 |
| Authors: | Vergara, Mauricio1 Becerra, Silvana2 Díaz Barrera, Alvaro1 Berrios, Julio1 Altamirano, Claudia1 |
| Institutions: | 1Pontificia Universidad Católica de Valparaíso, Escuela de Ingeniería Bioquímica, Valparaíso. Chile 2Centro Regional de Estudios en Alimentos Saludables, Valparaíso. Chile |
| Year: | 2012 |
| Volumen: | 15 |
| Number: | 6 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | We evaluated the combined effect of decreasing the temperature to a mild hypothermia range (34 and 31ºC) and switching to a slowly metabolizable carbon source (glucose substituted by galactose) on the growth and production of a recombinant human tissue plasminogen activator (rh-tPA) by Chinese hamster ovary cells in batch and semi-perfusion cultures. In batch cultures using glucose as a carbon source, decreasing the temperature caused a reduction in cell growth and an increase in specific productivity of rh-tPA of 32% at 34ºC and 55% at 31ºC, compared to cultures at 37ºC. Similar behaviour was observed in cultures at 34ºC using galactose as a carbon source. Nonetheless, at 31ºC, the specific productivity of rh-tPA strongly decreased (about 58%) compared to the culture at 37ºC. In semi-perfusion culture, the highest rh-tPA specific productivity was obtained at 34ºC. Similarly, whether a decrease in the temperature is accompanied of the replacement of glucose by galactose, the rh-tPA specific productivity improved about 112% over that obtained in semi-perfusion culture carried out at 37ºC with glucose as the carbon source. A semi-perfusion culture strategy was implemented based on the combined effect of the chosen carbon source and low temperatures, which was a useful approach for enhance the specific productivity of the recombinant protein |
| Disciplines: | Biología |
| Keyword: | Biotecnología, Cultivo de células, Células CHO, Hipotermia, Galactosa, Glutamato, Proteínas recombinantes |
| Keyword: | Biology, Biotechnology, Cell culture, CHO cells, Hypothermia, Galactose, Glutamate, Recombinant proteins |
| Full text: | Texto completo (Ver HTML) |
