| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000358470 |
| ISSN: | 0717-3458 |
| Authors: | Ni, Wei1 Hu, Shengwei1 Hazi, Wureli1 Wang, Yuanzhi1 Qiao, Jun1 Yan, Ren1 Chen, Chuangfu1 |
| Institutions: | 1Shihezi University, College of Animal Science and Technology, Shihezi. China |
| Year: | 2012 |
| Volumen: | 15 |
| Number: | 2 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Background: Gene silencing mediated by small interfering RNA (siRNA) has become a powerful biological tool for the regulation of gene expression. For the synthesis of siRNA by vector-based expression systems, several mammalian small nuclear RNA (snRNA) promoters have been cloned and shown different transcriptional efficiencies. Results: In this study, we identified a sheep 7SK snRNA (s7SK) promoter based on the highly conserved polymerase III promoter elements. Promoter activity was measured by promoter-driven shRNA expression to suppress expression of an exogenous reporter gene and endogenous sheep gene. Conclusions: The knock down assay demonstrated that the s7SK induced more stronger inhibition effect than human U6 and H1 promoters. The use of this native sheep 7SK promoter for shRNA expressionis an important component for development of RNAi-based gene therapy and production of transgenic animals in sheep species |
| Disciplines: | Medicina veterinaria y zootecnia |
| Keyword: | Ovinos y caprinos, Mejoramiento genético, Silenciamiento génico, ARN de interferencia, Ovejas |
| Keyword: | Veterinary medicine and animal husbandry, Sheep and goats, Genetic improvement, Gene silencing, Interference RNA, Sheep |
| Full text: | Texto completo (Ver HTML) |
