| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000267195 |
| ISSN: | 0717-3458 |
| Authors: | Leroy, Xavier J1 Leon, Karine Branchard, Michel |
| Institutions: | 1Universite de Bretagne Occidentale, Laboratoire de Biotechnologie et d’Amelioration des Plantes, Brest, Finistere. Francia |
| Year: | 2000 |
| Season: | Ago |
| Volumen: | 3 |
| Number: | 2 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Nota breve o noticia |
| Approach: | Experimental, aplicado |
| English abstract | This report describes a new application of the Inter-Simple Sequence Repeat (ISSR) technique. This technology based on the amplification of regions between microsatellites was applied to different calli from the same cauliflower mother plant. One of the tested ISSR primers, (GATA)4, generated great polymorphism. Twelve different markers were detected on polyacrylamide gels. After sequencing, one sequence showed homology with a predicated A. thaliana gene closely related to genes involved in the regulation of cell proliferation in mammalians. This marker is characterised by three microsatellites and a palindromic sequence. Possible causes of mutations in this marker are discussed and will be investigated. ISSR amplification appears as a reliable method in the determination of genetic instability at early stages in in vitro culture |
| Disciplines: | Agrociencias |
| Keyword: | Hortalizas, Biotecnología, Genética, Brassica oleracea, Col, Genoma, Microsatélites, Lesiones, ADN, Variación somaclonal |
| Keyword: | Agricultural sciences, Vegetables, Brassica oleracea, Cabbage, Genome, Lesions, DNA, Microsatellites, Somaclonal variation, Genetics |
| Full text: | Texto completo (Ver HTML) |
