| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000266098 |
| ISSN: | 0717-3458 |
| Authors: | Ali, Sikander1 Ikram-ul-Haq Qadeer, M.A |
| Institutions: | 1Government College, Department of Botany, Lahore, Punjab. Pakistán |
| Year: | 2002 |
| Season: | Ago |
| Volumen: | 5 |
| Number: | 2 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | L-DOPA is a useful drug for Parkinson’s disease. This investigation deals with the biosynthesis of L-DOPA by parental (GCB-6) and mutant (UV-7) strains of Aspergillus oryzae. There was a marked difference between the mycelial morphology and pellet type of the parental and UV-irradiated mutant cultures. The mutant strain of Aspergillus oryzae UV-6 showed pellet-like mycelial morphology and improved tyrosinase activity. Mould mycelium was used for biochemical conversion of L-tyrosine to L-DOPA since tyrosinase is an intracellular enzyme. The mutant was found to give 3.72 folds higher production of L-DOPA than the parental strain. The comparison of kinetic parameters was also done which showed greater ability of the mutant to yield L-DOPA (i.e. Yp/x 32.73 mg/mg with parent and 95.71 mg/mg with mutant). When cultures grown for various incubation periods, were monitored for Qp, Qs and qp, there was significant enhancement (p<0.0025-0.005) in these variables by the mutant strain of Aspergillus oryzae UV-7 over GCB-6 on all the rates |
| Disciplines: | Medicina, Biología |
| Keyword: | Microbiología, Hongos, Biotecnología, Aspergillus oryzae, L-dopa, Mutantes, Enfermedad de Parkinson |
| Keyword: | Medicine, Biology, Microbiology, Fungi, Aspergillus oryzae, L-dopa, Mutants, Parkinson disease |
| Full text: | Texto completo (Ver HTML) |
