Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Document title: Influence of the pH of glutaraldehyde and the use of dextran aldehyde on the preparation of cross-linked enzyme aggregates (CLEAs) of lipase from Burkholderia cepacia
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000398052
ISSN: 0717-3458
Authors: 1
1
1
Institutions: 1Pontificia Universidad Católica de Valparaíso, Facultad de Ingeniería, Valparaíso. Chile
Year:
Volumen: 14
Number: 3
Pages: 1-7
Country: Chile
Language: Inglés
Document type: Nota breve o noticia
Approach: Analítico, descriptivo
English abstract The preparation of cross -linked enzyme aggregates (CLEAs) of lipase has been a challenge due the low amount of lysine residues that lipases have on their surface. The results show that CLEAs prepared using dextran aldehyde (100- 200KDa) have a higher hydrolysis activity and particle size (activities between 3186 ± 21 U/g of CLEA and 4800 ± 30 U/g of CLEA and particle sizes between 52.6 ± 18.7 μm and 126.2 ± 53.5 μm) than CLEAs prepared with glutaraldehyde (0.1 KDa) (activities between 894 ± 16 U/g of CLEA and 2874 ± 20 U/g of CLEA and particle sizes between 21.2 ± 5.1 μm and 83.4 ± 24.9 μm); Thermal stability assays of bioctalysts at 60ºC at pH 7.0 using phosphate buffer 25 mM showed that CLEAs prepared with dextran aldehyde have lower residual activity after 50 hrs (maximum residual activity of 46.8% in the CLEA) than CLEAs prepared with glutaraldehyde (maximum residual activity of 70.2% in CLEA). When considering hydrolysis activity, thermal stability and residual activity of CLEAs as a criteria for selecting the best preparation conditions, it has been found that the best condition for CLEAs preparation are to use glutaraldehyde as cross -linking reagent at pH 9.5, at a concentration of 3.5 g/l, and an enzym e/albumin ratio of 15
Disciplines: Química,
Biología
Keyword: Bioquímica,
Bacterias,
Albúmina,
Dextrana,
Glutaraldehído,
Inmovilización,
Lipasas,
Entrecruzamiento,
Enzimas,
Burkholderia cepacia
Keyword: Chemistry,
Biology,
Biochemistry,
Bacteria,
Albumin,
Dextran,
Glutaraldehyde,
Immobilization,
Lipases,
Cross-linking,
Enzymes,
Burkholderia cepacia
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