Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
Document title: Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000398051
ISSN: 0717-3458
Authors: 1
2
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Institutions: 1Instituto Nacional de Tecnología Agropecuaria, Grupo de Biotecnología y Recursos Genéticos, Marcos Juárez, Córdoba. Argentina
2Instituto Nacional de Tecnología Agropecuaria, Area Fitopatología, Buenos Aires. Argentina
Year:
Volumen: 14
Number: 3
Pages: 1-17
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat ( Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resistance genes ( Lr genes) that may be present in a large group of wheat germplasm. Additionally presence of Lr genes can be determined using associated molecular markers. The objective of this study was to identify Lr genes that condition leaf rust resistance in 66 wheat cultivars from Argentina. Twenty four dif ferential lines with individual known leaf rust resistance genes were tested with 17 different pathotypes of leaf rust collected from Argentina. Leaf rust infection types produced on seedling plants of the 66 local cultivars were compared with the infection types produced by the same pathotypes on Lr differentials to postulate which seedling leaf rust genes were present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26, Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using molecular markers. Eleven different Lr genes were postulated in the material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26, Lr41 . Presence of Lr21 , Lr25 , Lr29 , and Lr47 could not be determined with the seventeen pathotypes used in the study because all were avirulent to these genes. Eleven cultivars (16.7%) were resistant to all pathotypes used in the study and the remaining 55 (83.3%) showed virulent reaction against one or more local pathotypes. Cultivars with seedl ing resistance gene combinations including Lr16 or single genes Lr47 (detected with molecular marker), Lr19 and Lr41 , showed high levels of resistance against all pathotypes or most of them. On the opposite side, cultivars with seedling resistance genes Lr 1 , Lr3a , Lr3a + Lr24 , Lr10 , Lr3a + Lr10 , Lr3a + Lr10 + L
Disciplines: Biología,
Agrociencias
Keyword: Hongos,
Fitopatología,
Roya foliar,
Puccinia triticina,
Trigo,
Triticum aestivum,
Control genético,
Patógenos,
Marcadores moleculares,
Fitomejoramiento,
Resistencia genética
Keyword: Biology,
Agricultural sciences,
Fungi,
Phytopathology,
Leaf rust,
Puccinia triticina,
Wheat,
Triticum aestivum,
Genetic control,
Pathogens,
Molecular markers,
Plant breeding,
Genetic resistance
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