Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay
Document title: Heat identification by 17β-estradiol and progesterone quantification in individual raw milk samples by enzyme immunoassay
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000358110
ISSN: 0717-3458
Authors: 1
2
3
2
1
1
Institutions: 1Instituto de Investigación y Tecnologías Agroalimentarias, Barcelona. España
2Parque Científico de Barcelona, Investigación Aplicada usando Ciencias Omicas, Barcelona. España
3Institución Catalana de Investigación y Estudios Avanzados, Barcelona. España
Year:
Volumen: 14
Number: 4
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Background: There is a substantial decline in first-service-pregnancy-rate in dairy cows. In this regard, future prospects are to measure milk hormones on-farm and progesterone levels in milk are not enough to precise ovulation unless connected to other data. The objectives of this study were to investigate whether 17β-estradiol could be measured from individual cow milk samples using a commercially available non-radiolabelled enzyme immunoassay kit (EIA) with no previously reported milk application, and whether those detections could precisely illustrate 17β-estradiol pre-ovulation peak in spite of its limited concentration and short manifestation in milk. Results: Milk sample treatments for progesterone and 17β-estradiol EIA measurements are described. Hormonal profiles from daily milk samples of six different cows were reported and 17β-estradiol pre-ovulation peak was visualized in all cases. Heat detection was possible by EIA using one every 2 days milking samples in almost all studied cases. Only in one case, morning and afternoon milking samples were required to visualize the 17β-estradiol pre-ovulation peak. Conclusions: 17β-estradiol EIA quantification in raw milk is a reliable, rapid, economic and a precise method to describe cow heat along with EIA progesterone determination
Disciplines: Medicina veterinaria y zootecnia
Keyword: Bovinos,
Medicina veterinaria,
Reproducción y genética animal,
Estro,
Marcadores bioquímicos,
Estradiol,
Progesterona,
Ganado lechero
Keyword: Veterinary medicine and animal husbandry,
Animal reproduction and genetics,
Bovines,
Veterinary medicine,
Estrous detection,
Biochemical markers,
Estradiol,
Progesterone,
Dairy cattle
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