| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000336149 |
| ISSN: | 0717-3458 |
| Authors: | Erisen, Semiha1 Yorgancilar, Mustafa2 Atalay, Emine2 Babaoglu, Mehmet2 Duran, Ahmet1 |
| Institutions: | 1Selcuk University, Ahmet Kelesoglu Education Faculty, Konya. Turquía 2Selcuk University, Faculty of Agriculture, Konya. Turquía |
| Year: | 2010 |
| Season: | Nov |
| Volumen: | 13 |
| Number: | 6 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | A callus induction and plant regeneration protocol was developed from leaf and petiole explants of the endemic Astragalus nezaketae. Explants were cultured on Murashige and Skoog medium (MS) supplemented with different plant growth regulators (PGRs) [α-naphthaleneacetic acid (NAA), benzyladenine (BA), 2,4-dichlorophenoxyacetic acid (2,4-D), kinetin (Kin), thidiazuron (TDZ)]. The combinations and concentrations of PGRs were shown significant variations for the frequency of callus formation, appearence of callus and the potential of callus differentiation. NAA x BA have been found highly affective in callusing and plant regeneration. Other PGRs have not resulted in callus differentiation for shoot formation. The highest number of shoots (6/explants) was obtained from leaf explants cultured on MS with 0.5 mg/l NAA and 4 mg/l BA. The regenerated shoots transferred to rooting medium (MS with 0.5 mg/l indole-3-butyric acid) were successfully rooted (100%) and showed rapid elongation. Rooted plantlets were acclimatized in pots containing 1:1 mixture of peat and perlite |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Fisiología vegetal, Cultivo de tejidos, Organogénesis, Regeneración vegetal, Astragalus nezaketae |
| Keyword: | Biology, Angiosperms, Plant physiology, Tissue culture, Organogenesis, Plant regeneration, Astragalus nezaketae |
| Full text: | Texto completo (Ver PDF) |
