| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000336063 |
| ISSN: | 0717-3458 |
| Authors: | Arcos Ortega, Guadalupe Fabiola1 Chan Kuuk, Rafael Antonio2 González Kantún, Wilma Aracely3 Souza Perera, Ramón2 Nakazawa Ueji, Yumi Elena3 Avilés Berzunza, Elidé3 Godoy Hernández, Gregorio3 Lawton, Michael A4 Zúñiga Aguilar, José Juan3 |
| Institutions: | 1Centro de Investigaciones Biológicas del Noroeste S.C., La Paz, Baja California Sur. México 2Instituto Tecnológico Superior de Calkiní, Calkiní, Campeche. México 3Centro de Investigación Científica de Yucatán A.C., Unidad de Bioquímica y Biología Molecular de Plantas, Mérida, Yucatán. México 4The State University of New Jersey, Biotechnological Center for the Agriculture and the Environment, New Brunswick, New Jersey. Estados Unidos de América |
| Year: | 2010 |
| Season: | Jul |
| Volumen: | 13 |
| Number: | 4 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Most of the pepper species of the genus Capsicum have been recalcitrant to efficient Agrobacterium tumefaciens-mediated stable or transient, genetic transformation. In the present work, we optimized a protocol for transient transformation of the Habanero pepper (Capsicum chinense Jacq.) through the standardization of several experimental factors. These included the age of the plants, the temperature, the length of co-cultivation, the application of a negative (vacuum) and/or a positive (infiltration) pressure, along with micro injection, the use of acetosyringone during the bacterial culturing, and modification of the pH during the GUS assay to eliminate the endogenous β- glucuronidase activity. The standardized protocol, which yielded nearly 55% fully transformed leaf explants, was used to successfully mobilize two empty binary vectors (pCAMBIA2301 and pCAMex), as well as the C. chinense cDNAs encoding the pathogenesisrelated protein 10 and esterase, respectively |
| Disciplines: | Biología |
| Keyword: | Biotecnología, Hortalizas, Transformación genética, Capsicum chinense, Agrobacterium tumefaciens |
| Keyword: | Biology, Biotechnology, Vegetables, Genetic transformation, Capsicum chinense, Agrobacterium tumefaciens |
| Full text: | Texto completo (Ver PDF) |
