| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000260386 |
| ISSN: | 0717-3458 |
| Authors: | Sarwat, Maryam1 Singh Negi, Madan2 Lakshmikumaran, Malathi Kumar Tyagi, Akhilesh3 Das, Sandip Shankar Srivastava, Prem |
| Institutions: | 1Jamia Hamdard University, Department of Biotechnology, Nueva Delhi, Delhi. India 2India Habitat Centre, Nueva Delhi, Delhi. India 3University of Delhi, Nueva Delhi, Delhi. India |
| Year: | 2006 |
| Season: | Ene |
| Volumen: | 9 |
| Number: | 1 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | For studying genetic diversity in natural populations of Terminalia, a medicinal plant, our attempts to isolate high quality DNA using several previously reported protocols and even modifications were unsuccessful. We therefore combined CTAB based isolation, and column based purification step, to isolate DNA from Terminalia arjuna. The DNA isolated using this standardized protocol was high in quality and suitable for restriction digestion and generation of random amplification of polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) |
| Disciplines: | Biología |
| Keyword: | Angiospermas, Genética, Biotecnología, Extracción química, ADN, Diversidad genética, Marcadores moleculares, Terminalia |
| Keyword: | Biology, Angiosperms, Genetics, Chemical extraction, DNA, Genetic diversity, Molecular markers, Terminalia |
| Full text: | Texto completo (Ver HTML) |
