| Journal: | Dermatología (México, D.F.) |
| Database: | PERIÓDICA |
| System number: | 000334884 |
| ISSN: | 0185-4038 |
| Authors: | Castrillón Rivera, Laura Estela1 Espinosa Antúnez, Violeta Karen1 Palma Ramos, Alejandro1 Padilla Desgarennes, María del Carmen2 |
| Institutions: | 1Universidad Autónoma Metropolitana, Departamento de Sistemas Biológicos, Xochimilco, Distrito Federal. México 2Centro Dermatológico Pascua, Laboratorio de Micología, México, Distrito Federal. México |
| Year: | 2011 |
| Season: | Ene-Feb |
| Volumen: | 55 |
| Number: | 1 |
| Pages: | 11-23 |
| Country: | México |
| Language: | Español |
| Document type: | Artículo |
| Approach: | Experimental |
| Spanish abstract | experimental para el desarrollo de biopelículas por este microorganismo |
| English abstract | Biofilms are bacterial communities that are the most successful form of colonization of microorganisms. They are considered as bacterial communities enclosed in a self-produced exopolymeric matrix and attached to an inert surface or a living tissue. Biofilm’s formation occurs as a continuous process with multiple steps of development; these steps are: conditioning, attachment, synthesis of extracellular matrix, maturation and dispersion, leading by surrounding oneself with the formation of a uniform structure like homogeneous deposits and viscous cell accumulation of a polymeric matrix with open channels for water movement. Objective: To assess different types of inert surfaces that allow the attachment as an initial phase in the biofilm’s formations by Nocardia brasiliensis, as well as its kinetics of formation on plastic, confirming its ability to form filaments. Material and method: Glasses and printer acetate of the trademarks PCM, Kronalin-E and Office Max were used as inert surfaces. Biophasic media was prepared in four boxes of glasses Petri in which were placed 10 mL of Agar Dextrose Sabouraud and broth Sabouraud with a concentration of 90 X 10 6 UFC/mL of Nocardia brasiliensis in each box. Results: Adherence was found in the three types of acetate, the best one was Office Max followed by the PCM and the Kronalin-E; the glass didn’t give good results. Discussion: Differences in the capacity of adherence and homogeneity were found depending of the kind of the material used. Besides the kinetic of adherence to plastic plate was gotten by these bacterial, which showed an increase of UFC/plate and filamentation of this colony and in these conditions. Conclusion: Adherence was achieved by N. brasiliensis into inert materials like acetate and plastics (boxes of disposable Petri); this is the first step for the experimental model in the development of biofilms by N. brasiliensis |
| Disciplines: | Medicina |
| Keyword: | Dermatología, Microbiología, Nocardia brasiliensis, Actinomicetos, Actinomicetoma, Biopelículas, Adherencia |
| Keyword: | Medicine, Dermatology, Microbiology, Actinomyces, Actinomycetoma, Biofilms, Adherence |
| Full text: | Texto completo (Ver PDF) |
